Mediation of sonic hedgehog-induced expression of COUP-TFII by a protein phosphatase

Venkatesh Krishnan, Fred A. Pereira, Yuhong Qiu, Chien Huan Chen, Philip A. Beachy, Sophia Y. Tsai, Ming Jer Tsai

Research output: Contribution to journalArticlepeer-review

129 Scopus citations

Abstract

A Sonic hedgehog (Shh) response element was identified in the chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII) promoter that binds to a factor distinct from Gli, a gene known to mediate Shh signaling. Although this binding activity is specifically stimulated by Shh-N (amino- terminal signaling domain), it can also be unmasked with protein phosphatase treatment in the mouse cell line P19, and induction by Shh-N can be blocked by phosphatase inhibitors. Thus, Shh-N signaling may result in dephosphorylation of a target factor that is required for activation of COUP- TFII-, Islet1-, and Gli response element-dependent gene expression. This finding identifies another step in the Shh-N signaling pathway.

Original languageEnglish
Pages (from-to)1947-1950
Number of pages4
JournalScience
Volume278
Issue number5345
DOIs
StatePublished - Dec 12 1997

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