TY - JOUR
T1 - Mechanisms responsible for cell volume regulation during hyperkalemic cardioplegic arrest
AU - Sun, Xiwu
AU - Ducko, Christopher T.
AU - Hoenicke, Eric M.
AU - Reigle, Karen
AU - Damiano, Ralph J.
N1 - Funding Information:
This study was supported by NIH grant HL-51032 (Ralph J. Damiano, Jr) and NIH NRSA grant HL-09925 (Christopher T. Ducko, Ralph J. Damiano, Jr).
PY - 2000
Y1 - 2000
N2 - Background. Cardioplegia has been shown to induce significant cell swelling. This study tested the hypothesis that (1) the [K+][Cl-] product of the cardioplegia solution is the main determinant of myocyte swelling, and (2) reperfusion myocyte shrinkage results from a rectifying Cl- conductance. Methods. Rabbit ventricular myocytes were superfused with 37°C Krebs-Henseleit solution for 10 minutes. Then cells underwent 20 minutes of superfusion with standard St. Thomas' solution ([K+][Cl-] product = 2566 mmol/L2) and two solutions with lower [K+][Cl-] product (1500 and 700 mmol/L2) at 9°C. Cells were then resuperfused with 37°C Krebs-Henseleit solution for 30 minutes. Cell volume was measured by videomicroscopy. Results. Cells superfused with St. Thomas' having [K+][Cl-] products of 2,566, 1,500, and 700 mmol/L2 swelled by 9.18% ± 3.57%, 5.51% ± 1.08%, and 1.49% ± 1.56%, respectively. Reexposure to Krebs-Henseleit solution caused these cells to shrink by 5.79% ± 1.41%, 8.72% ± 3.68%, and 13.46% ± 5.60%, respectively. This shrinkage was blocked by Cl- channel blockers given at the onset of superfusion. Conclusions. Lowering the [K+][Cl-] product of St. Thomas' solution attenuated myocyte edema. Myocyte shrinkage during reexposure to Krebs-Henseleit solution resulted from the volume-activated Cl- channel.(C) 2000 by The Society of Thoracic Surgeons.
AB - Background. Cardioplegia has been shown to induce significant cell swelling. This study tested the hypothesis that (1) the [K+][Cl-] product of the cardioplegia solution is the main determinant of myocyte swelling, and (2) reperfusion myocyte shrinkage results from a rectifying Cl- conductance. Methods. Rabbit ventricular myocytes were superfused with 37°C Krebs-Henseleit solution for 10 minutes. Then cells underwent 20 minutes of superfusion with standard St. Thomas' solution ([K+][Cl-] product = 2566 mmol/L2) and two solutions with lower [K+][Cl-] product (1500 and 700 mmol/L2) at 9°C. Cells were then resuperfused with 37°C Krebs-Henseleit solution for 30 minutes. Cell volume was measured by videomicroscopy. Results. Cells superfused with St. Thomas' having [K+][Cl-] products of 2,566, 1,500, and 700 mmol/L2 swelled by 9.18% ± 3.57%, 5.51% ± 1.08%, and 1.49% ± 1.56%, respectively. Reexposure to Krebs-Henseleit solution caused these cells to shrink by 5.79% ± 1.41%, 8.72% ± 3.68%, and 13.46% ± 5.60%, respectively. This shrinkage was blocked by Cl- channel blockers given at the onset of superfusion. Conclusions. Lowering the [K+][Cl-] product of St. Thomas' solution attenuated myocyte edema. Myocyte shrinkage during reexposure to Krebs-Henseleit solution resulted from the volume-activated Cl- channel.(C) 2000 by The Society of Thoracic Surgeons.
UR - http://www.scopus.com/inward/record.url?scp=0033840577&partnerID=8YFLogxK
U2 - 10.1016/S0003-4975(00)01403-X
DO - 10.1016/S0003-4975(00)01403-X
M3 - Article
C2 - 10969692
AN - SCOPUS:0033840577
VL - 70
SP - 633
EP - 638
JO - Annals of Thoracic Surgery
JF - Annals of Thoracic Surgery
SN - 0003-4975
IS - 2
ER -