Mechanisms of gene regulation in human embryos and pluripotent stem cells

Thorold W. Theunissen, Rudolf Jaenisch

Research output: Contribution to journalReview article

27 Scopus citations

Abstract

Pluripotent stem cells have broad utility in biomedical research and their molecular regulation has thus garnered substantial interest. While the principles that establish and regulate pluripotency have been well defined in the mouse, it has been difficult to extrapolate these insights to the human system due to species-specific differences and the distinct developmental identities of mouse versus human embryonic stem cells. In this Review, we examine genome-wide approaches to elucidate the regulatory principles of pluripotency in human embryos and stem cells, and highlight where differences exist in the regulation of pluripotency in mice and humans. We review recent insights into the nature of human pluripotent cells in vivo, obtained by the deep sequencing of pre-implantation embryos. We also present an integrated overview of the principal layers of global gene regulation in human pluripotent stem cells. Finally, we discuss the transcriptional and epigenomic remodeling events associated with cell fate transitions into and out of human pluripotency.

Original languageEnglish
Pages (from-to)4496-4509
Number of pages14
JournalDevelopment (Cambridge)
Volume144
Issue number24
DOIs
StatePublished - Dec 15 2017

Keywords

  • Chromatin
  • Embryogenesis
  • Epigenetics
  • Pluripotency
  • Reprogramming
  • Transcription

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