TY - JOUR
T1 - Mechanisms of Broad-Band UVB Irradiation‒Induced Itch in Mice
AU - Cao, Liang
AU - Yue, Xueping
AU - Zhao, Yonghui
AU - Du, Lixia
AU - Xie, Zili
AU - Yuan, Yi
AU - Zhang, Sha
AU - Li, Feng
AU - Feng, Jing
AU - Hu, Hongzhen
N1 - Funding Information:
This work was supported partly by grants from the National Institutes of Health , R01AA027065, R01AR077183, and R01DK103901 (to HH.), Center for the Study of Itch & Sensory Disorders, and the Department of Anesthesiology at Washington University School of Medicine in St. Louis to HH. The work was performed at the Department of Anesthesiology, Center for the Study of Itch & Sensory Disorders, Washington University School of Medicine in St. Louis (St. Louis, MO).
Funding Information:
This work was supported partly by grants from the National Institutes of Health, R01AA027065, R01AR077183, and R01DK103901 (to HH.), Center for the Study of Itch & Sensory Disorders, and the Department of Anesthesiology at Washington University School of Medicine in St. Louis to HH. The work was performed at the Department of Anesthesiology, Center for the Study of Itch & Sensory Disorders, Washington University School of Medicine in St. Louis (St. Louis, MO). Conceptualization: HH, JF; Data Curation: LC, XY, YZ, LD; Formal Analysis: LC, XY, YZ, LD, YY, SZ, FL; Funding Acquisition: HH; Investigation: LC, XY, YZ, LD, SZ; Methodology: LC, XY, YZ, LD; Project Administration: HH, JF; Resources: HH; Supervision: HH, JF; Writing - Original Draft Preparation: JF, LC, HH; Writing - Review and Editing: JF, LC, HH.
Publisher Copyright:
© 2021 The Authors
PY - 2021/10
Y1 - 2021/10
N2 - Although sunburn can produce severe uncontrollable itching, the underlying mechanisms of UV irradiation‒induced itch are poorly understood because of a lack of experimental animal models of sunburn itch. In this study, we established a sunburn-related mouse model and found that broad-band UVB irradiation elicited scratching but not wiping behavior in mice. Using a combination of live-cell calcium ion imaging and quantitative RT-PCR on dorsal root ganglion neurons, H&E staining, immunofluorescence staining of skin preparations, and behavioral testing, in combination with genetic and pharmacological approaches, we showed that TRPV1-positive dorsal root ganglion neurons but not mast cells are involved in broad-band UVB irradiation‒induced itch. Moreover, both genetic and pharmacological inhibition of TRPV1 function significantly alleviated the broad-band UVB irradiation‒induced itch response. Collectively, our results suggest that broad-band UVB irradiation evokes itch sensation in mice by promoting TRPV1 channel function in dorsal root ganglion neurons and provide potential therapeutic targets for sunburn-related itch.
AB - Although sunburn can produce severe uncontrollable itching, the underlying mechanisms of UV irradiation‒induced itch are poorly understood because of a lack of experimental animal models of sunburn itch. In this study, we established a sunburn-related mouse model and found that broad-band UVB irradiation elicited scratching but not wiping behavior in mice. Using a combination of live-cell calcium ion imaging and quantitative RT-PCR on dorsal root ganglion neurons, H&E staining, immunofluorescence staining of skin preparations, and behavioral testing, in combination with genetic and pharmacological approaches, we showed that TRPV1-positive dorsal root ganglion neurons but not mast cells are involved in broad-band UVB irradiation‒induced itch. Moreover, both genetic and pharmacological inhibition of TRPV1 function significantly alleviated the broad-band UVB irradiation‒induced itch response. Collectively, our results suggest that broad-band UVB irradiation evokes itch sensation in mice by promoting TRPV1 channel function in dorsal root ganglion neurons and provide potential therapeutic targets for sunburn-related itch.
UR - http://www.scopus.com/inward/record.url?scp=85106370335&partnerID=8YFLogxK
U2 - 10.1016/j.jid.2021.03.015
DO - 10.1016/j.jid.2021.03.015
M3 - Article
C2 - 33812858
AN - SCOPUS:85106370335
SN - 0022-202X
VL - 141
SP - 2499-2508.e3
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
IS - 10
ER -