Mechanism of regulation of intrachromatid recombination and long-range chromosome interactions in Saccharomyces cerevisiae

Shamsu Zaman, Malay Choudhury, James C. Jiang, Pankaj Srivastava, Bidyut K. Mohanty, Christopher Danielson, Sean J. Humphrey, S. Michal Jazwinski, Deepak Bastia

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

The NAD-dependent histone deacetylase Sir2 controls ribosomal DNA (rDNA) silencing by inhibiting recombination and RNA polymerase II-catalyzed transcription in the rDNA of Saccharomyces cerevisiae. Sir2 is recruited to nontranscribed spacer 1 (NTS1) of the rDNA array by interaction between the RENT (regulation of nucleolar silencing and telophase exit) complex and the replication terminator protein Fob1. The latter binds to its cognate sites, called replication termini (Ter) or replication fork barriers (RFB), that are located in each copy of NTS1. This work provides new mechanistic insights into the regulation of rDNA silencing and intrachromatid recombination by showing that Sir2 recruitment is stringently regulated by Fob1 phosphorylation at specific sites in its C-terminal domain (C-Fob1), which also regulates long-range Ter-Ter interactions. We show further that long-range Fob1-mediated Ter-Ter interactions in trans are downregulated by Sir2. These regulatory mechanisms control intrachromatid recombination and the replicative life span (RLS).

Original languageEnglish
Pages (from-to)1451-1463
Number of pages13
JournalMolecular and cellular biology
Volume36
Issue number10
DOIs
StatePublished - May 1 2016

Fingerprint

Dive into the research topics of 'Mechanism of regulation of intrachromatid recombination and long-range chromosome interactions in Saccharomyces cerevisiae'. Together they form a unique fingerprint.

Cite this