Mechanism-based inactivation of dopamine β-monooxygenase in adrenal chromaffin cells

Sheldon W. May, Frederick K. Young, Jennifer L. Powers, Michelle M. Gill-Woznichak

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Dopamine β-monooxygenase (DBM, E.C. 1.14.17.1) is an attractive target point for possible modulation of adrenergic activity, and a variety of DBM-targeted pseudosabstrates and inhibitors have been developed in this laboratory and other laboratories. We now demonstrate the efficacy of a DBM-targeted mechanism-based inactivator, as well as enzymatic processing of two alternate DBM substrates, within functional adrenal chromaffin cells. When cultured adrenal medullary chromaffin cells were incubated with the mecha nism-based inactivator 1-(4'-hydroxyphenyl)-1-(aminomethyl)-ethene (HOPAME), vesicular DBM activity was markedly decreased. Similarly, the alternate substrates 4'-hydroxyphenyl-2-aminoethyl sulfide and 4'-hydroxyphenyl-2-aminopropyl selenide each undergo uptake and DBM-catalyzed oxygenation within these cells. The simultaneous action of both the mechanism-based inactivator and an alternate substrate within functional chromaffin cells was also demonstrated. These results provide support for a direct mechanistic link between the enzymological properties of DBM-targeted adrenergic agents and their in-vivo pharmacological activities.

Original languageEnglish
Pages (from-to)278-284
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume228
Issue number2
DOIs
StatePublished - Nov 12 1996
Externally publishedYes

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