Mechanics of cell spreading: Role of myosin II

Tetsuro Wakatsuki, Robert B. Wysolmerski, Elliot L. Elson

Research output: Contribution to journalReview article

117 Scopus citations

Abstract

As it migrates over substratum, a cell must exert different kinds of forces that act at various cellular locations and at specific times. These forces must therefore be coordinately regulated. The Rho-family GTPases Rac1 and Cdc42 promote actin polymerization that drives extension of the leading cell edge. Subsequently, RhoA regulates myosin-dependent contractile force, which is required for formation of adhesive contacts and stress fibers. During cell spreading, however, the activity of RhoA is reduced by a mechanism involving the tyrosine kinases c-Src and focal adhesion kinase (FAK), and the p190RhoGAP. It has been proposed that this reduction of RhoA activity facilitates edge extension by reducing myosin-dependent contractile forces that could resist this process. We have directly tested this hypothesis by correlating myosin activity with the rate of cell spreading on a substratum. The rate of spreading is inversely related to the myosin activity. Furthermore, spreading is inhibited by low concentrations of cytochalasin D, as expected for a process that depends on the growth of uncapped actin filaments. Cell indentation measurements show that a myosin-dependent viscoelastic force resists cell deformation.

Original languageEnglish
Pages (from-to)1617-1625
Number of pages9
JournalJournal of cell science
Volume116
Issue number8
DOIs
StatePublished - Apr 15 2003

Keywords

  • Cell mechanics
  • Cell spreading
  • Contraction
  • Cytochalasin D
  • Myosin II
  • Traction force

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