Mechanics-driven nuclear localization of YAP can be reversed by N-cadherin ligation in mesenchymal stem cells

Cheng Zhang, Hongyuan Zhu, Xinru Ren, Bin Gao, Bo Cheng, Shaobao Liu, Baoyong Sha, Zhaoqing Li, Zheng Zhang, Yi Lv, Haohua Wang, Hui Guo, Tian Jian Lu, Feng Xu, Guy M. Genin, Min Lin

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

Mesenchymal stem cells adopt differentiation pathways based upon cumulative effects of mechanosensing. A cell’s mechanical microenvironment changes substantially over the course of development, beginning from the early stages in which cells are typically surrounded by other cells and continuing through later stages in which cells are typically surrounded by extracellular matrix. How cells erase the memory of some of these mechanical microenvironments while locking in memory of others is unknown. Here, we develop a material and culture system for modifying and measuring the degree to which cells retain cumulative effects of mechanosensing. Using this system, we discover that effects of the RGD adhesive motif of fibronectin (representative of extracellular matrix), known to impart what is often termed “mechanical memory” in mesenchymal stem cells via nuclear YAP localization, are erased by the HAVDI adhesive motif of the N-cadherin (representative of cell-cell contacts). These effects can be explained by a motor clutch model that relates cellular traction force, nuclear deformation, and resulting nuclear YAP re-localization. Results demonstrate that controlled storage and removal of proteins associated with mechanical memory in mesenchymal stem cells is possible through defined and programmable material systems.

Original languageEnglish
Article number6229
JournalNature communications
Volume12
Issue number1
DOIs
StatePublished - Dec 1 2021

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