Measuring the kinetic and mechanical properties of non-processive myosins using optical tweezers

Michael J. Greenberg, Henry Shuman, E. Michael Ostap

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

6 Scopus citations

Abstract

The myosin superfamily of molecular motors utilizes energy from ATP hydrolysis to generate force and motility along actin filaments in a diverse array of cellular processes. These motors are structurally, kinetically, and mechanically tuned to their specific molecular roles in the cell. Optical trapping techniques have played a central role in elucidating the mechanisms by which myosins generate force and in exposing the remarkable diversity of myosin functions. Here, we present thorough methods for measuring and analyzing interactions between actin and non-processive myosins using optical trapping techniques.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages483-509
Number of pages27
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1486
ISSN (Print)1064-3745

Keywords

  • Actomyosin
  • Kinetics
  • Mechanochemistry
  • Single molecule

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