@inbook{2367915c699e4fdcab75f8db120e99ac,
title = "Measuring the kinetic and mechanical properties of non-processive myosins using optical tweezers",
abstract = "The myosin superfamily of molecular motors utilizes energy from ATP hydrolysis to generate force and motility along actin filaments in a diverse array of cellular processes. These motors are structurally, kinetically, and mechanically tuned to their specific molecular roles in the cell. Optical trapping techniques have played a central role in elucidating the mechanisms by which myosins generate force and in exposing the remarkable diversity of myosin functions. Here, we present thorough methods for measuring and analyzing interactions between actin and non-processive myosins using optical trapping techniques.",
keywords = "Actomyosin, Kinetics, Mechanochemistry, Single molecule",
author = "Greenberg, {Michael J.} and Henry Shuman and Ostap, {E. Michael}",
note = "Funding Information: The authors wish to acknowledge grants from the National Institutes of Health (R01GM057247 and P01GM087253 to E.M.O. and R00HL123623 to M.J.G.). Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2017.",
year = "2017",
doi = "10.1007/978-1-4939-6421-5_19",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "483--509",
booktitle = "Methods in Molecular Biology",
}