Measuring the 3D position and orientation of single molecules simultaneously and accurately with the double helix microscope

Matthew D. Lew; Mikael P. Backlund; Adam S. Backer; Steffen J. Sahl; Ginni Grover; Anurag Agrawal; Rafael Piestun; W. E. Moerner

doi:10.1364/cleo_si.2013.ctu3n.3

Measuring the 3D position and orientation of single molecules simultaneously and accurately with the double helix microscope

Matthew D. Lew, Mikael P. Backlund, Adam S. Backer, Steffen J. Sahl, Ginni Grover, Anurag Agrawal, Rafael Piestun, W. E. Moerner

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Ignoring the anisotropic nature of single-fluorophore emission in super-resolution microscopy can lead to large localization errors. We use the Double-Helix microscope to correct such mislocalizations while simultaneously extracting 3D position and molecular orientation.

Original language	English
Title of host publication	2013 Conference on Lasers and Electro-Optics, CLEO 2013
Publisher	IEEE Computer Society
ISBN (Print)	9781557529725
DOIs	https://doi.org/10.1364/cleo_si.2013.ctu3n.3
State	Published - 2013
Event	2013 Conference on Lasers and Electro-Optics, CLEO 2013 - San Jose, CA, United States Duration: Jun 9 2013 → Jun 14 2013

Publication series

Name	2013 Conference on Lasers and Electro-Optics, CLEO 2013

Conference

Conference	2013 Conference on Lasers and Electro-Optics, CLEO 2013
Country/Territory	United States
City	San Jose, CA
Period	06/9/13 → 06/14/13

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10.1364/cleo_si.2013.ctu3n.3

Cite this

Lew, M. D., Backlund, M. P., Backer, A. S., Sahl, S. J., Grover, G., Agrawal, A., Piestun, R., & Moerner, W. E. (2013). Measuring the 3D position and orientation of single molecules simultaneously and accurately with the double helix microscope. In 2013 Conference on Lasers and Electro-Optics, CLEO 2013 Article 6833636 (2013 Conference on Lasers and Electro-Optics, CLEO 2013). IEEE Computer Society. https://doi.org/10.1364/cleo_si.2013.ctu3n.3

@inproceedings{c23aaa274025480998a7acc3f9c63509,

title = "Measuring the 3D position and orientation of single molecules simultaneously and accurately with the double helix microscope",

abstract = "Ignoring the anisotropic nature of single-fluorophore emission in super-resolution microscopy can lead to large localization errors. We use the Double-Helix microscope to correct such mislocalizations while simultaneously extracting 3D position and molecular orientation.",

author = "Lew, {Matthew D.} and Backlund, {Mikael P.} and Backer, {Adam S.} and Sahl, {Steffen J.} and Ginni Grover and Anurag Agrawal and Rafael Piestun and Moerner, {W. E.}",

year = "2013",

doi = "10.1364/cleo_si.2013.ctu3n.3",

language = "English",

isbn = "9781557529725",

series = "2013 Conference on Lasers and Electro-Optics, CLEO 2013",

publisher = "IEEE Computer Society",

booktitle = "2013 Conference on Lasers and Electro-Optics, CLEO 2013",

note = "2013 Conference on Lasers and Electro-Optics, CLEO 2013 ; Conference date: 09-06-2013 Through 14-06-2013",

}

Lew, MD, Backlund, MP, Backer, AS, Sahl, SJ, Grover, G, Agrawal, A, Piestun, R & Moerner, WE 2013, Measuring the 3D position and orientation of single molecules simultaneously and accurately with the double helix microscope. in 2013 Conference on Lasers and Electro-Optics, CLEO 2013., 6833636, 2013 Conference on Lasers and Electro-Optics, CLEO 2013, IEEE Computer Society, 2013 Conference on Lasers and Electro-Optics, CLEO 2013, San Jose, CA, United States, 06/9/13. https://doi.org/10.1364/cleo_si.2013.ctu3n.3

Measuring the 3D position and orientation of single molecules simultaneously and accurately with the double helix microscope. / Lew, Matthew D.; Backlund, Mikael P.; Backer, Adam S. et al.
2013 Conference on Lasers and Electro-Optics, CLEO 2013. IEEE Computer Society, 2013. 6833636 (2013 Conference on Lasers and Electro-Optics, CLEO 2013).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Measuring the 3D position and orientation of single molecules simultaneously and accurately with the double helix microscope

AU - Lew, Matthew D.

AU - Backlund, Mikael P.

AU - Backer, Adam S.

AU - Sahl, Steffen J.

AU - Grover, Ginni

AU - Agrawal, Anurag

AU - Piestun, Rafael

AU - Moerner, W. E.

PY - 2013

Y1 - 2013

N2 - Ignoring the anisotropic nature of single-fluorophore emission in super-resolution microscopy can lead to large localization errors. We use the Double-Helix microscope to correct such mislocalizations while simultaneously extracting 3D position and molecular orientation.

AB - Ignoring the anisotropic nature of single-fluorophore emission in super-resolution microscopy can lead to large localization errors. We use the Double-Helix microscope to correct such mislocalizations while simultaneously extracting 3D position and molecular orientation.

UR - http://www.scopus.com/inward/record.url?scp=84903760370&partnerID=8YFLogxK

U2 - 10.1364/cleo_si.2013.ctu3n.3

DO - 10.1364/cleo_si.2013.ctu3n.3

M3 - Conference contribution

AN - SCOPUS:84903760370

SN - 9781557529725

T3 - 2013 Conference on Lasers and Electro-Optics, CLEO 2013

BT - 2013 Conference on Lasers and Electro-Optics, CLEO 2013

PB - IEEE Computer Society

T2 - 2013 Conference on Lasers and Electro-Optics, CLEO 2013

Y2 - 9 June 2013 through 14 June 2013

ER -

Lew MD, Backlund MP, Backer AS, Sahl SJ, Grover G, Agrawal A et al. Measuring the 3D position and orientation of single molecules simultaneously and accurately with the double helix microscope. In 2013 Conference on Lasers and Electro-Optics, CLEO 2013. IEEE Computer Society. 2013. 6833636. (2013 Conference on Lasers and Electro-Optics, CLEO 2013). doi: 10.1364/cleo_si.2013.ctu3n.3

Measuring the 3D position and orientation of single molecules simultaneously and accurately with the double helix microscope

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