Measuring rotational dynamics with high accuracy and precision using a tri-spot point spread function

Oumeng Zhang, Matthew D. Lew

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Fluorescence microscopy is limited to measuring even-order moments of dipole orientation, thereby causing bias when estimating molecular rotational dynamics. We designed a Tri-spot PSF that measures rotational constraint accurately and precisely.

Original languageEnglish
Title of host publicationNovel Techniques in Microscopy - Proceedings Biophotonics Congress
Subtitle of host publicationOptics in the Life Sciences Congress 2019 (BODA, BRAIN, NTM, OMA, OMP)
PublisherOptical Society of America (OSA)
ISBN (Electronic)9781943580545
DOIs
StatePublished - Apr 8 2019
EventNovel Techniques in Microscopy, NTM 2019 - Part of Biophotonics Congress: Optics in the Life Sciences Congress 2019 - Tucson, United States
Duration: Apr 14 2019Apr 17 2019

Publication series

NameNovel Techniques in Microscopy - Proceedings Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA, BRAIN, NTM, OMA, OMP)

Conference

ConferenceNovel Techniques in Microscopy, NTM 2019 - Part of Biophotonics Congress: Optics in the Life Sciences Congress 2019
Country/TerritoryUnited States
CityTucson
Period04/14/1904/17/19

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