@inproceedings{ee889e686d3f402a83ef48fee5633dde,
title = "Measuring 3D molecular orientation and rotational mobility using a Tri-spot point spread function",
abstract = "We present a method to measure the molecular orientation and rotational mobility of single-molecule emitters by designing and implementing a Tri-spot point spread function. It can measure all degrees of freedom related to molecular orientation and rotational mobility. Its design is optimized by maximizing the theoretical limit of its measurement precision. We evaluate the precision and accuracy of the Tri-spot PSF by measuring the orientation and effective rotational mobility of single fluorescent molecules embedded in a polymer matrix.",
keywords = "fluorescence microscopy, phase mask design, point spread function engineering, single-molecule imaging",
author = "Oumeng Zhang and Tianben Ding and Jin Lu and Hesam Mazidi and Lew, {Matthew D.}",
note = "Publisher Copyright: {\textcopyright} COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.; Single Molecule Spectroscopy and Superresolution Imaging XI 2018 ; Conference date: 27-01-2018 Through 28-01-2018",
year = "2018",
doi = "10.1117/12.2287004",
language = "English",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Felix Koberling and Ingo Gregor and Jorg Enderlein and Rainer Erdmann and Gryczynski, {Zygmunt Karol}",
booktitle = "Single Molecule Spectroscopy and Superresolution Imaging XI",
}