Measurement of upregulation of inducible nitric oxide synthase in the experimental autoimmune encephalomyelitis model using a positron emitting radiopharmaceutical

Jian Zhang; Anne H. Cross; Timothy J. McCarthy; Michael J. Welch

doi:10.1006/niox.1997.0120

Measurement of upregulation of inducible nitric oxide synthase in the experimental autoimmune encephalomyelitis model using a positron emitting radiopharmaceutical

Jian Zhang, Anne H. Cross, Timothy J. McCarthy, Michael J. Welch

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13 Scopus citations

Abstract

Excess nitric oxide has been implicated in the pathogenosis of experimental autoimmune encephalomyelitis (EAE) which is an animal model for multiple sclerosis. Positron emission tomography (PET) is an imaging technique that has shown utility for studying enzyme systems in vivo. A positron-labeled inducible nitric oxide synthetase (iNOS) inhibitor has been studied in EAE-affected mice as well as controls. Greater uptake of the radiolabeled inhibitor was observed in the spinal cord of the affected mice than of control mice. Increased uptake was also observed in other organs not previously implicated in this experimental model. The increased up-take of the radiopharmaceutical in this model suggests that this tracer may have the potential for measuring increased levels of iNOS in humans by PET.

Original language	English
Pages (from-to)	263-267
Number of pages	5
Journal	Nitric Oxide - Biology and Chemistry
Volume	1
Issue number	3
DOIs	https://doi.org/10.1006/niox.1997.0120
State	Published - Jun 1997

Keywords

Encephalomyelitis
Enzyme systems
Multiple sclerosis
Nitric oxide synthase
Positron emission tomography (PET)
Radiopharmaceutical

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10.1006/niox.1997.0120

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title = "Measurement of upregulation of inducible nitric oxide synthase in the experimental autoimmune encephalomyelitis model using a positron emitting radiopharmaceutical",

abstract = "Excess nitric oxide has been implicated in the pathogenosis of experimental autoimmune encephalomyelitis (EAE) which is an animal model for multiple sclerosis. Positron emission tomography (PET) is an imaging technique that has shown utility for studying enzyme systems in vivo. A positron-labeled inducible nitric oxide synthetase (iNOS) inhibitor has been studied in EAE-affected mice as well as controls. Greater uptake of the radiolabeled inhibitor was observed in the spinal cord of the affected mice than of control mice. Increased uptake was also observed in other organs not previously implicated in this experimental model. The increased up-take of the radiopharmaceutical in this model suggests that this tracer may have the potential for measuring increased levels of iNOS in humans by PET.",

keywords = "Encephalomyelitis, Enzyme systems, Multiple sclerosis, Nitric oxide synthase, Positron emission tomography (PET), Radiopharmaceutical",

author = "Jian Zhang and Cross, {Anne H.} and McCarthy, {Timothy J.} and Welch, {Michael J.}",

note = "Funding Information: This work was supported by NIH (HL13851) to M.J.W. and RG2681 from the National Mutiple Sclerosis Society to A.H.C. The authors thank Bill Margenau and David Ficke for isotope production and Manuel San and Elizabeth Sherman for assistance with the animal model.",

year = "1997",

month = jun,

doi = "10.1006/niox.1997.0120",

language = "English",

volume = "1",

pages = "263--267",

journal = "Nitric Oxide - Biology and Chemistry",

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AU - Zhang, Jian

AU - Cross, Anne H.

AU - McCarthy, Timothy J.

AU - Welch, Michael J.

N1 - Funding Information: This work was supported by NIH (HL13851) to M.J.W. and RG2681 from the National Mutiple Sclerosis Society to A.H.C. The authors thank Bill Margenau and David Ficke for isotope production and Manuel San and Elizabeth Sherman for assistance with the animal model.

PY - 1997/6

Y1 - 1997/6

N2 - Excess nitric oxide has been implicated in the pathogenosis of experimental autoimmune encephalomyelitis (EAE) which is an animal model for multiple sclerosis. Positron emission tomography (PET) is an imaging technique that has shown utility for studying enzyme systems in vivo. A positron-labeled inducible nitric oxide synthetase (iNOS) inhibitor has been studied in EAE-affected mice as well as controls. Greater uptake of the radiolabeled inhibitor was observed in the spinal cord of the affected mice than of control mice. Increased uptake was also observed in other organs not previously implicated in this experimental model. The increased up-take of the radiopharmaceutical in this model suggests that this tracer may have the potential for measuring increased levels of iNOS in humans by PET.

AB - Excess nitric oxide has been implicated in the pathogenosis of experimental autoimmune encephalomyelitis (EAE) which is an animal model for multiple sclerosis. Positron emission tomography (PET) is an imaging technique that has shown utility for studying enzyme systems in vivo. A positron-labeled inducible nitric oxide synthetase (iNOS) inhibitor has been studied in EAE-affected mice as well as controls. Greater uptake of the radiolabeled inhibitor was observed in the spinal cord of the affected mice than of control mice. Increased uptake was also observed in other organs not previously implicated in this experimental model. The increased up-take of the radiopharmaceutical in this model suggests that this tracer may have the potential for measuring increased levels of iNOS in humans by PET.

KW - Encephalomyelitis

KW - Enzyme systems

KW - Multiple sclerosis

KW - Nitric oxide synthase

KW - Positron emission tomography (PET)

KW - Radiopharmaceutical

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U2 - 10.1006/niox.1997.0120

DO - 10.1006/niox.1997.0120

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AN - SCOPUS:0031408099

SN - 1089-8603

VL - 1

SP - 263

EP - 267

JO - Nitric Oxide - Biology and Chemistry

JF - Nitric Oxide - Biology and Chemistry

IS - 3

ER -

Measurement of upregulation of inducible nitric oxide synthase in the experimental autoimmune encephalomyelitis model using a positron emitting radiopharmaceutical

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