Measurement of Inositol Trisphosphate by Gas Chromatography/Mass Spectrometry: Femtomole Sensitivity Provided by Negative-Ion Chemical Ionization Mass Spectrometry in Submilligram Quantities of Tissue

Leona J. Rubin, Fong Fu Hsu, William R. Sherman

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Using NICI GC/MS with selected ion monitoring, we have extended the limit of detection for inositol to the femtomole level with a high degree of chemical specificity. The high sensitivity of the method and the simplified procedures for tissue extraction and dephosphorylation allow us to measure quantitatively levels of any of the inositol polyphosphates from submilligram quantities of tissue. With the recent developments of ion chromatography HPLC (which provides good separation of inositol polyphosphate isomers) and ion suppression procedures in which the inositol phosphates elute in water it should be possible, with our method, to measure the amounts of many of the individual inositol polyphosphates in small amounts of tissue.

Original languageEnglish
Title of host publicationMethods in Neurosciences
Pages201-212
Number of pages12
EditionC
DOIs
StatePublished - Jan 1 1993

Publication series

NameMethods in Neurosciences
NumberC
Volume18
ISSN (Print)1043-9471

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