Abstract

The goal of this study is to develop an in vitro assay for elastin expression using RFL6 cells, which were derived from fibroblasts present in rat lung tissue, and to test different doses of minoxidil and diazoxide, potent vasodilators and potassium channel openers. Elastin amounts in response to drug treatments are assessed using a commercial Fastin Elastin assay. Our project will shed light on optimal pharmaceutical doses for increasing elastin expression and also provide a relatively high throughput method for testing additional anti-hypertensive drugs that may affect elastin expression and subsequently decrease arterial stiffness and blood pressure.Grant: Acknowledgements: Funding by the AMGEN Scholars Program, NIH grants HL105314 and HL115560 and Washington University in St. Louis are gratefully acknowledged.

Original languageEnglish
Title of host publicationStudent Poster Sessions 2014 - Core Programming Area at the 2014 AIChE Annual Meeting
PublisherAIChE
Pages181-182
Number of pages2
ISBN (Electronic)9781510812734
StatePublished - 2014
EventStudent Poster Sessions 2014 - Core Programming Area at the 2014 AIChE Annual Meeting - Atlanta, United States
Duration: Nov 16 2014Nov 21 2014

Publication series

NameStudent Poster Sessions 2014 - Core Programming Area at the 2014 AIChE Annual Meeting

Conference

ConferenceStudent Poster Sessions 2014 - Core Programming Area at the 2014 AIChE Annual Meeting
Country/TerritoryUnited States
CityAtlanta
Period11/16/1411/21/14

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