Measurement of elastin, collagen, and total protein levels in tissues

Ivan Stoilov, Barry C. Starcher, Robert P. Mecham, Thomas J. Broekelmann

Research output: Chapter in Book/Report/Conference proceedingChapter

20 Scopus citations

Abstract

Elastin and collagen levels in tissues are frequently difficult to measure because of each protein's limited solubility. This chapter provides detailed methodology for the determination of elastin, collagen, and total protein levels in a single tissue sample. All three assays start with an acid hydrolysate of the tissue, which breaks the tissue-associated proteins down to their component amino acids. Marker amino acids unique to each protein (desmosine for elastin and hydroxyproline for collagen) are then quantified. Total protein content, useful as a denominator for data normalization, can also be measured from a portion of the hydrolysate using an assay for free amino groups. These measurements are performed using convenient 96-well assay plates and require only a plate reader to determine absorbance.

Original languageEnglish
Title of host publicationMethods in Cell Biology
PublisherAcademic Press Inc.
Pages133-146
Number of pages14
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Cell Biology
Volume143
ISSN (Print)0091-679X

Keywords

  • Collagen
  • Desmosine
  • ELISA
  • Elastin
  • Hydroxyproline

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  • Cite this

    Stoilov, I., Starcher, B. C., Mecham, R. P., & Broekelmann, T. J. (2018). Measurement of elastin, collagen, and total protein levels in tissues. In Methods in Cell Biology (pp. 133-146). (Methods in Cell Biology; Vol. 143). Academic Press Inc.. https://doi.org/10.1016/bs.mcb.2017.08.008