Measurement of DNA damage in mammalian cells exposed in vitro to radiofrequency fields at SARs of 3-5 W/kg

L. Li, K. S. Bisht, I. LaGroye, P. Zhang, W. L. Straube, E. G. Moros, J. L. Roti Roti

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

In the present study, we determined whether exposure of mammalian cells to 3.2-5.1 W/kg specific absorption rate (SAR) radiofrequency fields could induce DNA damage in murine C3H 10T1/2 fibroblasts. Cell cultures were exposed to 847.74 MHz code-division multiple access (CDMA) and 835.62 frequency-division multiple access (FDMA) modulated radiations in radial transmission line (RTL) irradiators in which the temperature was regulated to 37.0 ± 0.3°C. Using the alkaline comet assay to measure DNA damage, we found no statistically significant differences in either comet moment or comet length between sham-exposed cells and those exposed for 2, 4 or 24 h to CDMA or FDMA radiations in either exponentially growing or plateau-phase cells. Further, a 4-h incubation after the 2-h exposure resulted in no significant changes in comet moment or comet length. Our results show that exposure of cultured C3H 10T1/2 cells at 37°C CDMA or FDMA at SAR values of up to 5.1 W/kg did not induce measurable DNA damage.

Original languageEnglish
Pages (from-to)328-332
Number of pages5
JournalRadiation research
Volume156
Issue number3
DOIs
StatePublished - Jan 1 2001

Fingerprint

Dive into the research topics of 'Measurement of DNA damage in mammalian cells exposed in vitro to radiofrequency fields at SARs of 3-5 W/kg'. Together they form a unique fingerprint.

Cite this