TY - JOUR
T1 - Measurement of cyclosporine concentrations in whole blood
T2 - HPLC and radioimmunoassay with a specific monoclonal antibody and 3H- or 125I-labeled ligand compared
AU - Wolf, B. A.
AU - Daft, M. C.
AU - Koenig, J. W.
AU - Flye, M. W.
AU - Turk, J. W.
AU - Scott, M. G.
PY - 1989
Y1 - 1989
N2 - We compared cyclosporine concentrations in whole blood as measured by HPLC and by RIA with a monoclonal antibody specific for cyclosporine with 3H- or 125I-labeled cyclosporine ligand. The 3H-RIA kit slightly underestimated cyclosporine concentrations (>600 μg/L) in comparison with HPLC. Over a wide range of concentrations, cyclosporine measured with the 125I-RIA kit correlated well with HPLC (slope = 0.99, n = 301, r = 0.98), observed for samples from recipients of kidney, heart, or liver allografts (respective slopes: 1.01, 0.93, and 1.00). The 125I-RIA standard curve was linear to 1000 μg of cyclosporine per liter. Inter- and intra-assay CVs for 125I-RIA measurements of cyclosporine were ≤7%. Evidently, the 125I-RIA kit involving a monoclonal antibody specific for cyclosporine is equivalent to the HPLC assay and can replace it for therapeutic drug monitoring of cyclosporine therapy.
AB - We compared cyclosporine concentrations in whole blood as measured by HPLC and by RIA with a monoclonal antibody specific for cyclosporine with 3H- or 125I-labeled cyclosporine ligand. The 3H-RIA kit slightly underestimated cyclosporine concentrations (>600 μg/L) in comparison with HPLC. Over a wide range of concentrations, cyclosporine measured with the 125I-RIA kit correlated well with HPLC (slope = 0.99, n = 301, r = 0.98), observed for samples from recipients of kidney, heart, or liver allografts (respective slopes: 1.01, 0.93, and 1.00). The 125I-RIA standard curve was linear to 1000 μg of cyclosporine per liter. Inter- and intra-assay CVs for 125I-RIA measurements of cyclosporine were ≤7%. Evidently, the 125I-RIA kit involving a monoclonal antibody specific for cyclosporine is equivalent to the HPLC assay and can replace it for therapeutic drug monitoring of cyclosporine therapy.
UR - https://www.scopus.com/pages/publications/0024557610
U2 - 10.1093/clinchem/35.1.120
DO - 10.1093/clinchem/35.1.120
M3 - Article
C2 - 2642760
AN - SCOPUS:0024557610
SN - 0009-9147
VL - 35
SP - 120
EP - 124
JO - Clinical chemistry
JF - Clinical chemistry
IS - 1
ER -