Measurement of cyclosporine concentrations in whole blood: HPLC and radioimmunoassay with a specific monoclonal antibody and 3H- or 125I-labeled ligand compared

B. A. Wolf, M. C. Daft, J. W. Koenig, M. W. Flye, J. W. Turk, M. G. Scott

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26 Scopus citations

Abstract

We compared cyclosporine concentrations in whole blood as measured by HPLC and by RIA with a monoclonal antibody specific for cyclosporine with 3H- or 125I-labeled cyclosporine ligand. The 3H-RIA kit slightly underestimated cyclosporine concentrations (>600 μg/L) in comparison with HPLC. Over a wide range of concentrations, cyclosporine measured with the 125I-RIA kit correlated well with HPLC (slope = 0.99, n = 301, r = 0.98), observed for samples from recipients of kidney, heart, or liver allografts (respective slopes: 1.01, 0.93, and 1.00). The 125I-RIA standard curve was linear to 1000 μg of cyclosporine per liter. Inter- and intra-assay CVs for 125I-RIA measurements of cyclosporine were ≤7%. Evidently, the 125I-RIA kit involving a monoclonal antibody specific for cyclosporine is equivalent to the HPLC assay and can replace it for therapeutic drug monitoring of cyclosporine therapy.

Original languageEnglish
Pages (from-to)120-124
Number of pages5
JournalClinical chemistry
Volume35
Issue number1
StatePublished - Jan 1 1989

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