Measurement of cyclosporine concentrations in whole blood: HPLC and radioimmunoassay with a specific monoclonal antibody and 3H- or 125I-labeled ligand compared

B. A. Wolf; M. C. Daft; J. W. Koenig; M. W. Flye; J. W. Turk; M. G. Scott

Measurement of cyclosporine concentrations in whole blood: HPLC and radioimmunoassay with a specific monoclonal antibody and ³H- or ¹²⁵I-labeled ligand compared

B. A. Wolf, M. C. Daft, J. W. Koenig, M. W. Flye, J. W. Turk, M. G. Scott

Research output: Contribution to journal › Article › peer-review

Abstract

We compared cyclosporine concentrations in whole blood as measured by HPLC and by RIA with a monoclonal antibody specific for cyclosporine with ³H- or ¹²⁵I-labeled cyclosporine ligand. The ³H-RIA kit slightly underestimated cyclosporine concentrations (>600 μg/L) in comparison with HPLC. Over a wide range of concentrations, cyclosporine measured with the ¹²⁵I-RIA kit correlated well with HPLC (slope = 0.99, n = 301, r = 0.98), observed for samples from recipients of kidney, heart, or liver allografts (respective slopes: 1.01, 0.93, and 1.00). The ¹²⁵I-RIA standard curve was linear to 1000 μg of cyclosporine per liter. Inter- and intra-assay CVs for ¹²⁵I-RIA measurements of cyclosporine were ≤7%. Evidently, the ¹²⁵I-RIA kit involving a monoclonal antibody specific for cyclosporine is equivalent to the HPLC assay and can replace it for therapeutic drug monitoring of cyclosporine therapy.

Original language	English
Pages (from-to)	120-124
Number of pages	5
Journal	Clinical chemistry
Volume	35
Issue number	1
State	Published - Jan 1 1989

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title = "Measurement of cyclosporine concentrations in whole blood: HPLC and radioimmunoassay with a specific monoclonal antibody and 3H- or 125I-labeled ligand compared",

abstract = "We compared cyclosporine concentrations in whole blood as measured by HPLC and by RIA with a monoclonal antibody specific for cyclosporine with 3H- or 125I-labeled cyclosporine ligand. The 3H-RIA kit slightly underestimated cyclosporine concentrations (>600 μg/L) in comparison with HPLC. Over a wide range of concentrations, cyclosporine measured with the 125I-RIA kit correlated well with HPLC (slope = 0.99, n = 301, r = 0.98), observed for samples from recipients of kidney, heart, or liver allografts (respective slopes: 1.01, 0.93, and 1.00). The 125I-RIA standard curve was linear to 1000 μg of cyclosporine per liter. Inter- and intra-assay CVs for 125I-RIA measurements of cyclosporine were ≤7%. Evidently, the 125I-RIA kit involving a monoclonal antibody specific for cyclosporine is equivalent to the HPLC assay and can replace it for therapeutic drug monitoring of cyclosporine therapy.",

author = "Wolf, {B. A.} and Daft, {M. C.} and Koenig, {J. W.} and Flye, {M. W.} and Turk, {J. W.} and Scott, {M. G.}",

year = "1989",

month = jan,

day = "1",

language = "English",

volume = "35",

pages = "120--124",

journal = "Clinical Chemistry",

issn = "0009-9147",

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Measurement of cyclosporine concentrations in whole blood : HPLC and radioimmunoassay with a specific monoclonal antibody and ³H- or ¹²⁵I-labeled ligand compared. / Wolf, B. A.; Daft, M. C.; Koenig, J. W.; Flye, M. W.; Turk, J. W.; Scott, M. G.

In: Clinical chemistry, Vol. 35, No. 1, 01.01.1989, p. 120-124.

Research output: Contribution to journal › Article › peer-review

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AU - Daft, M. C.

AU - Koenig, J. W.

AU - Flye, M. W.

AU - Turk, J. W.

AU - Scott, M. G.

PY - 1989/1/1

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N2 - We compared cyclosporine concentrations in whole blood as measured by HPLC and by RIA with a monoclonal antibody specific for cyclosporine with 3H- or 125I-labeled cyclosporine ligand. The 3H-RIA kit slightly underestimated cyclosporine concentrations (>600 μg/L) in comparison with HPLC. Over a wide range of concentrations, cyclosporine measured with the 125I-RIA kit correlated well with HPLC (slope = 0.99, n = 301, r = 0.98), observed for samples from recipients of kidney, heart, or liver allografts (respective slopes: 1.01, 0.93, and 1.00). The 125I-RIA standard curve was linear to 1000 μg of cyclosporine per liter. Inter- and intra-assay CVs for 125I-RIA measurements of cyclosporine were ≤7%. Evidently, the 125I-RIA kit involving a monoclonal antibody specific for cyclosporine is equivalent to the HPLC assay and can replace it for therapeutic drug monitoring of cyclosporine therapy.

AB - We compared cyclosporine concentrations in whole blood as measured by HPLC and by RIA with a monoclonal antibody specific for cyclosporine with 3H- or 125I-labeled cyclosporine ligand. The 3H-RIA kit slightly underestimated cyclosporine concentrations (>600 μg/L) in comparison with HPLC. Over a wide range of concentrations, cyclosporine measured with the 125I-RIA kit correlated well with HPLC (slope = 0.99, n = 301, r = 0.98), observed for samples from recipients of kidney, heart, or liver allografts (respective slopes: 1.01, 0.93, and 1.00). The 125I-RIA standard curve was linear to 1000 μg of cyclosporine per liter. Inter- and intra-assay CVs for 125I-RIA measurements of cyclosporine were ≤7%. Evidently, the 125I-RIA kit involving a monoclonal antibody specific for cyclosporine is equivalent to the HPLC assay and can replace it for therapeutic drug monitoring of cyclosporine therapy.

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Measurement of cyclosporine concentrations in whole blood: HPLC and radioimmunoassay with a specific monoclonal antibody and ³H- or ¹²⁵I-labeled ligand compared

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