Measurement of cyclic nucleotides in histologically defined samples of brain and retina

J. A. Ferrendelli, E. H. Rubin, H. T. Orr, D. A. Kinscherf, O. H. Lowry

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

A method is described which allows measurement of cyclic AMP and cyclic GMP in histologically defined tissue samples containing as little as 0.6 fmol of either compound. This permits, for example, assays of freeze-dried samples as small as 0.03 μg dry weight from individual retina layers or 4-μg samples from individual layers of cerebellum. The procedure involves a modified radioimmunoassay at reduced volume for acetylated cyclic nucleotides in combination with some of the techniques previously developed for quantitative histochemical assays.

Original languageEnglish
Pages (from-to)252-259
Number of pages8
JournalAnalytical Biochemistry
Volume78
Issue number1
DOIs
StatePublished - Mar 1977

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