Measurement of cerebral vascular extraction fractions in the rat using intracarotid injection techniques

H. Brent Clark, Hartman Boyd K., Raichle Marcus E., Preskorn Sheldon H., Kenneth B. Larson

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

A small volume (5 μl) common carotid arterial injection method is described for the quantitation of cerebral vascular extraction fractions (Et) of diffusion limited tracer molecules in the rat. The method is a modification of a technique diffusion duced by Oldendorf and widely used for the study of blood-brain barrier phenomena. While the Oldendorf technique has proven valuable for estimating the relative permeabilities of substances, it is limited in measuring Et under conditions of physiologically or pharmacologically altered permeability or blood flow. The method described in this paper - using a small volume (5 μl) common carotid injection, a freely diffusible reference tracer, [14C]butanol, and a 5 sec circulation time - allows for measurements of Et that reflect changes in blood flow and small differences in permeability. The modified method is important for the study of the regulation of cerebral vascular permeability and flow in an inexpensive animal model.

Original languageEnglish
Pages (from-to)311-323
Number of pages13
JournalBrain Research
Volume208
Issue number2
DOIs
StatePublished - Mar 16 1981
Externally publishedYes

Keywords

  • butanol
  • carotid artery
  • cerebrovascular permeability
  • diffusion limitation
  • extraction fraction
  • indicator diffusion
  • water

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