Measurement and significance of antibodies against GM1ganglioside Report of a workshop, 18 April 1989, Chicago, IL, U.S.A.

Donald M. Marcus; Norman Latov; Bart P. Hsi; Baiba K. Gillard; Jack Antel; Stanley Appel; Lanfranco Callegaro; Genevieve Daune; Norman Latoy; Donald Marcus; Eduardo Nobile-Orazio; Alan Pestronk; Jacques Portoukalian; Richard Quarles; Edgar Salazar-Grueso; Larry Schneider; Michael Shy; Andreas Steck

doi:10.1016/0165-5728(89)90144-6

Measurement and significance of antibodies against G_M1ganglioside Report of a workshop, 18 April 1989, Chicago, IL, U.S.A.

Donald M. Marcus, Norman Latov, Bart P. Hsi, Baiba K. Gillard, Jack Antel, Stanley Appel, Lanfranco Callegaro, Genevieve Daune, Norman Latoy, Donald Marcus, Eduardo Nobile-Orazio, Alan Pestronk, Jacques Portoukalian, Richard Quarles, Edgar Salazar-Grueso, Larry Schneider, Michael Shy, Andreas Steck

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Abstract

Twelve laboratories from the United States, Canada, France, Italy and Switzerland participated in a workshop to compare assay used to measure anti-G_M1antibodies, and to discuss the clinical significance of these antibodies. A panel of test samples containing varying amounts of anti-G_M1antibody was prepared by mixing varied proportions of normal serum with a serum containing a monoclonal IgM antibody that bound G_M1ganglioside. Enzyme-linked immunosorbent assay (ELISA) data were supplied by eight laboratories and ten laboratories classified the sera as negative, weakly of strongly positive. Most laboratories correctly identified the two samples that contained the highest quantities of antibody, but there was considerable disagreement on the classification of the three samples with moderate or small.

Original language	English
Pages (from-to)	255-259
Number of pages	5
Journal	Journal of Neuroimmunology
Volume	25
Issue number	2-3
DOIs	https://doi.org/10.1016/0165-5728(89)90144-6
State	Published - Jan 1 1989

Keywords

Anti-ganglioside
Enzyme-linked immunosorbent assay
Neuropathy

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10.1016/0165-5728(89)90144-6

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Marcus, D. M., Latov, N., Hsi, B. P., Gillard, B. K., Antel, J., Appel, S., Callegaro, L., Daune, G., Latoy, N., Marcus, D., Nobile-Orazio, E., Pestronk, A., Portoukalian, J., Quarles, R., Salazar-Grueso, E., Schneider, L., Shy, M., & Steck, A. (1989). Measurement and significance of antibodies against G_M1ganglioside Report of a workshop, 18 April 1989, Chicago, IL, U.S.A. Journal of Neuroimmunology, 25(2-3), 255-259. https://doi.org/10.1016/0165-5728(89)90144-6

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abstract = "Twelve laboratories from the United States, Canada, France, Italy and Switzerland participated in a workshop to compare assay used to measure anti-GM1antibodies, and to discuss the clinical significance of these antibodies. A panel of test samples containing varying amounts of anti-GM1antibody was prepared by mixing varied proportions of normal serum with a serum containing a monoclonal IgM antibody that bound GM1ganglioside. Enzyme-linked immunosorbent assay (ELISA) data were supplied by eight laboratories and ten laboratories classified the sera as negative, weakly of strongly positive. Most laboratories correctly identified the two samples that contained the highest quantities of antibody, but there was considerable disagreement on the classification of the three samples with moderate or small.",

keywords = "Anti-ganglioside, Enzyme-linked immunosorbent assay, Neuropathy",

author = "Marcus, {Donald M.} and Norman Latov and Hsi, {Bart P.} and Gillard, {Baiba K.} and Jack Antel and Stanley Appel and Lanfranco Callegaro and Genevieve Daune and Norman Latoy and Donald Marcus and Eduardo Nobile-Orazio and Alan Pestronk and Jacques Portoukalian and Richard Quarles and Edgar Salazar-Grueso and Larry Schneider and Michael Shy and Andreas Steck",

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month = jan,

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doi = "10.1016/0165-5728(89)90144-6",

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Marcus, DM, Latov, N, Hsi, BP, Gillard, BK, Antel, J, Appel, S, Callegaro, L, Daune, G, Latoy, N, Marcus, D, Nobile-Orazio, E, Pestronk, A, Portoukalian, J, Quarles, R, Salazar-Grueso, E, Schneider, L, Shy, M & Steck, A 1989, 'Measurement and significance of antibodies against G_M1ganglioside Report of a workshop, 18 April 1989, Chicago, IL, U.S.A.', Journal of Neuroimmunology, vol. 25, no. 2-3, pp. 255-259. https://doi.org/10.1016/0165-5728(89)90144-6

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AU - Marcus, Donald M.

AU - Latov, Norman

AU - Hsi, Bart P.

AU - Gillard, Baiba K.

AU - Antel, Jack

AU - Appel, Stanley

AU - Callegaro, Lanfranco

AU - Daune, Genevieve

AU - Latoy, Norman

AU - Marcus, Donald

AU - Nobile-Orazio, Eduardo

AU - Pestronk, Alan

AU - Portoukalian, Jacques

AU - Quarles, Richard

AU - Salazar-Grueso, Edgar

AU - Schneider, Larry

AU - Shy, Michael

AU - Steck, Andreas

PY - 1989/1/1

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N2 - Twelve laboratories from the United States, Canada, France, Italy and Switzerland participated in a workshop to compare assay used to measure anti-GM1antibodies, and to discuss the clinical significance of these antibodies. A panel of test samples containing varying amounts of anti-GM1antibody was prepared by mixing varied proportions of normal serum with a serum containing a monoclonal IgM antibody that bound GM1ganglioside. Enzyme-linked immunosorbent assay (ELISA) data were supplied by eight laboratories and ten laboratories classified the sera as negative, weakly of strongly positive. Most laboratories correctly identified the two samples that contained the highest quantities of antibody, but there was considerable disagreement on the classification of the three samples with moderate or small.

AB - Twelve laboratories from the United States, Canada, France, Italy and Switzerland participated in a workshop to compare assay used to measure anti-GM1antibodies, and to discuss the clinical significance of these antibodies. A panel of test samples containing varying amounts of anti-GM1antibody was prepared by mixing varied proportions of normal serum with a serum containing a monoclonal IgM antibody that bound GM1ganglioside. Enzyme-linked immunosorbent assay (ELISA) data were supplied by eight laboratories and ten laboratories classified the sera as negative, weakly of strongly positive. Most laboratories correctly identified the two samples that contained the highest quantities of antibody, but there was considerable disagreement on the classification of the three samples with moderate or small.

KW - Anti-ganglioside

KW - Enzyme-linked immunosorbent assay

KW - Neuropathy

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ER -

Measurement and significance of antibodies against G_M1ganglioside Report of a workshop, 18 April 1989, Chicago, IL, U.S.A.

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