TY - JOUR
T1 - MCF-10A-NeoST
T2 - A new cell system for studying cell-ECM and cell-cell interactions in breast cancer
AU - Dodge Zantek, Nicole
AU - Walker-Daniels, Jennifer
AU - Stewart, Jane
AU - Hansen, Rhonda K.
AU - Robinson, Daniel
AU - Miao, Hui
AU - Wang, Bingcheng
AU - Kung, Hsing Jien
AU - Bissell, Mina J.
AU - Kinch, Michael S.
PY - 2001
Y1 - 2001
N2 - Purpose: There is a continuing need for genetically matched cell systems to model cellular behaviors that are frequently observed in aggressive breast cancers. Experimental Design: We report here the isolation and initial characterization of a spontaneously arising variant of MCF-10A cells, NeoST, which provides a new model to study cell adhesion and signal transduction in breast cancer. Results: NeoST cells recapitulate important biological and biochemical features of metastatic breast cancer, including anchorage-independent growth, invasiveness in three-dimensional reconstituted membranes, loss of E-cadherin expression, and increased tyrosine kinase activity. A comprehensive analysis of tyrosine kinase expression revealed overexpression or functional activation of the Axl, FAK, and EphA2 tyrosine kinases in transformed MCF-10A cells. Conclusions: MCF-10A and these new derivatives provide a genetically matched model to study defects in cell adhesion and signaling that are relevant to cellular behaviors that often typify aggressive breast cancer cells.
AB - Purpose: There is a continuing need for genetically matched cell systems to model cellular behaviors that are frequently observed in aggressive breast cancers. Experimental Design: We report here the isolation and initial characterization of a spontaneously arising variant of MCF-10A cells, NeoST, which provides a new model to study cell adhesion and signal transduction in breast cancer. Results: NeoST cells recapitulate important biological and biochemical features of metastatic breast cancer, including anchorage-independent growth, invasiveness in three-dimensional reconstituted membranes, loss of E-cadherin expression, and increased tyrosine kinase activity. A comprehensive analysis of tyrosine kinase expression revealed overexpression or functional activation of the Axl, FAK, and EphA2 tyrosine kinases in transformed MCF-10A cells. Conclusions: MCF-10A and these new derivatives provide a genetically matched model to study defects in cell adhesion and signaling that are relevant to cellular behaviors that often typify aggressive breast cancer cells.
UR - http://www.scopus.com/inward/record.url?scp=0035175828&partnerID=8YFLogxK
M3 - Article
C2 - 11705887
AN - SCOPUS:0035175828
SN - 1078-0432
VL - 7
SP - 3640
EP - 3648
JO - Clinical Cancer Research
JF - Clinical Cancer Research
IS - 11
ER -