Matriptase sensitive gold nanoparticle probe for early cancer detection

Dongyin Zhang, Jianpeng Xue, Junmei Tian, Jing Jin, Jie Cao, Sisi Cui, Samuel Achilefu, Yueqing Gu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Matriptase is a type II transmembrane serine protease highly expressed on the surface of tumor cells and participated in a range of human cancers including those of the breast, cervix, ovaries, prostate, colon and gastrointestinal tract. In this study, we developed a new matriptase detection probe with gold nanoparticles (AuNPs). A fluorescence dye was attached to AuNP surface through the bridge of peptide substrate (GRQSRAC). The fluorescence was quenched due to the fluorescence resonance energy transfer (FRET) between AuNP and the fluorescence dye. The fluorescence recovery properties were confirmed by dithiothreitol in vitro. The result of in vitro cell research indicates that the gold nanoparticle probe is sensitive and specificitive for the detection of matriptase expression in early tumor and has great potential in biomedical application.

Original languageEnglish
Title of host publicationOptical, Electronic Materials and Applications II
Pages301-305
Number of pages5
DOIs
StatePublished - Oct 29 2012
Event2nd International Conference on Optical, Electronic Materials and Applications 2012, OEMA 2012 - Chongqing, China
Duration: May 25 2012May 26 2012

Publication series

NameAdvanced Materials Research
Volume529
ISSN (Print)1022-6680

Conference

Conference2nd International Conference on Optical, Electronic Materials and Applications 2012, OEMA 2012
CountryChina
CityChongqing
Period05/25/1205/26/12

Keywords

  • FRET
  • Gold nanoparticle
  • Matriptase

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  • Cite this

    Zhang, D., Xue, J., Tian, J., Jin, J., Cao, J., Cui, S., Achilefu, S., & Gu, Y. (2012). Matriptase sensitive gold nanoparticle probe for early cancer detection. In Optical, Electronic Materials and Applications II (pp. 301-305). (Advanced Materials Research; Vol. 529). https://doi.org/10.4028/www.scientific.net/AMR.529.301