Mast cell migration and chemotaxis assayed by microscopy

Monika Bambousková, Zuzana Rubíková, Lubica Dráberová, Pavel Dráber, Petr Dráber

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


A better understanding of the molecular mechanisms leading to mast cell migration and chemotaxis is the long-term goal in mast cell research and is essential for comprehension of mast cell function in health and disease. Various techniques have been developed in recent decades for in vitro and in vivo assessment of mast cell motility and chemotaxis. In this chapter, three microscopy assays facilitating real-time quantification of mast cell chemotaxis and migration are described, focusing on individual cell tracking and data analysis.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages18
StatePublished - 2020

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Cell migration
  • Cell tracking
  • Chemoattractant
  • Chemokine
  • Chemotaxis
  • Mast cells


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