TY - JOUR
T1 - Mass spectrometry-based shotgun lipidomics – a critical review from the technical point of view
AU - Hsu, Fong Fu
N1 - Funding Information:
This work is supported by US Public Health Service Grants P41-GM103422, P60-DK-20579, P30-DK56341, 4R33HL120760-03 and R01AI130454. The author acknowledges Dr. Cheryl Frankfater and the reviewers for proofreading of the manuscript. The author declares no conflict of interest.
Publisher Copyright:
© 2018, Springer-Verlag GmbH Germany, part of Springer Nature.
PY - 2018/10/1
Y1 - 2018/10/1
N2 - Over the past decade, mass spectrometry (MS)-based “shotgun lipidomics” has emerged as a powerful tool for quantitative and qualitative analysis of the complex lipids in the biological system. The aim of this critical review is to give the interested reader a concise overview of the current state of the technology, focused on lipidomic analysis by mass spectrometry. The pros and cons, and pitfalls associated with each available “shotgun lipidomics” method are discussed; and the new strategies for improving the current methods are described. A list of important papers and reviews that are sufficient rather than comprehensive, covering all the aspects of lipidomics including the workflow, methodology, and fundamentals is also compiled for readers to follow. [Figure not available: see fulltext.].
AB - Over the past decade, mass spectrometry (MS)-based “shotgun lipidomics” has emerged as a powerful tool for quantitative and qualitative analysis of the complex lipids in the biological system. The aim of this critical review is to give the interested reader a concise overview of the current state of the technology, focused on lipidomic analysis by mass spectrometry. The pros and cons, and pitfalls associated with each available “shotgun lipidomics” method are discussed; and the new strategies for improving the current methods are described. A list of important papers and reviews that are sufficient rather than comprehensive, covering all the aspects of lipidomics including the workflow, methodology, and fundamentals is also compiled for readers to follow. [Figure not available: see fulltext.].
KW - Collision induced dissociation
KW - Electrospray ionization
KW - Lipid
KW - Lipidomics
KW - MALDI
KW - Mass spectrometry
UR - http://www.scopus.com/inward/record.url?scp=85051639737&partnerID=8YFLogxK
U2 - 10.1007/s00216-018-1252-y
DO - 10.1007/s00216-018-1252-y
M3 - Review article
C2 - 30094786
AN - SCOPUS:85051639737
SN - 1618-2642
VL - 410
SP - 6387
EP - 6409
JO - Analytical and Bioanalytical Chemistry
JF - Analytical and Bioanalytical Chemistry
IS - 25
ER -