TY - JOUR
T1 - Mapping quantitative trait loci for open-field behavior in mice
AU - Gershenfeld, Howard K.
AU - Neumann, Paul E.
AU - Mathis, Chantal
AU - Crawley, Jacqueline N.
AU - Li, Xiaohua
AU - Paul, Steven M.
N1 - Funding Information:
H.G. would like to thank C. B. Taylor, I. L. Weissman, and D. Baltimore for encouragement in the larval stages of this project. We appreciated helpful conversations with E. Remmers, J. Thomas, E. Ginns, R. Philibert, W. Dietrich, E. Lander, C. Basten, and Z. B. Zeng, our NIH and UTSW colleagues. We are grateful to the NIMH Computer support group, the Whitehead/MIT Mouse Genome Project, Research Genetics, and the Mouse Genome Informatics Project providing infrastructure for this work. This project has been generously supported by NIMH-LRP, a NARSAD Young Investigators Award (H.G.), the Southwestern Medical Foundation (H.G.), the KZA Hope Fund (H.G.), and the Medical Research Council of Canada (P.E.N.).
PY - 1997
Y1 - 1997
N2 - By performing a whole genome screen in an F2 intercross of two strains of mice (A/J and C57BL/6J), which differ markedly in their behavioral response to a brightly lit open field (O-F) we have mapped several quantitative trait loci (QTL) for this complex behavioral phenotype. QTL on chromosomes 1 and 10 were identified that affect both initial ambulation in the O-F (initial 'response to novelty' ambulation) (lod of 7.1 and 8.8, respectively) and vertical rearings (lod of 4.5 and 8.5, respectively). For habituated O-F behavior, QTL were identified on chromosomes 3 and 10 for ambulation (lod of 4.1 and 14.7, respectively) and on chromosomes 1, 10, and 19 for vertical rearings (lod of 5.8, 6.0, and 4.7, respectively). The QTL on chromosome 1 (near D1Mit116; 101 cM) was specific for initial O-F ambulation behavior, whereas the QTL on chromosome 10 (near D10Mit237; 74 cM) affected both initial and habituated rearing behavior. Additional suggestive QTL (lod, >2.8) were mapped to chromosomes 1, 8, 11, 15, and 19. The QTL on chromosomes 1, 10, and 19 individually explain from 3.2 to 12.7%. Collectively, the multiple independent QTL explain from 16.3 to 24.1% of the F2 population's phenotypic variance, depending on the trait. These identified QTL should prove useful for dissecting the genetic and behavioral dimensions of O-F behavior, fostering an understanding of individual differences.
AB - By performing a whole genome screen in an F2 intercross of two strains of mice (A/J and C57BL/6J), which differ markedly in their behavioral response to a brightly lit open field (O-F) we have mapped several quantitative trait loci (QTL) for this complex behavioral phenotype. QTL on chromosomes 1 and 10 were identified that affect both initial ambulation in the O-F (initial 'response to novelty' ambulation) (lod of 7.1 and 8.8, respectively) and vertical rearings (lod of 4.5 and 8.5, respectively). For habituated O-F behavior, QTL were identified on chromosomes 3 and 10 for ambulation (lod of 4.1 and 14.7, respectively) and on chromosomes 1, 10, and 19 for vertical rearings (lod of 5.8, 6.0, and 4.7, respectively). The QTL on chromosome 1 (near D1Mit116; 101 cM) was specific for initial O-F ambulation behavior, whereas the QTL on chromosome 10 (near D10Mit237; 74 cM) affected both initial and habituated rearing behavior. Additional suggestive QTL (lod, >2.8) were mapped to chromosomes 1, 8, 11, 15, and 19. The QTL on chromosomes 1, 10, and 19 individually explain from 3.2 to 12.7%. Collectively, the multiple independent QTL explain from 16.3 to 24.1% of the F2 population's phenotypic variance, depending on the trait. These identified QTL should prove useful for dissecting the genetic and behavioral dimensions of O-F behavior, fostering an understanding of individual differences.
KW - Chromosomal mapping
KW - Exploratory behavior
KW - Habituation
KW - Novelty
KW - Quantitative trait loci (QTL)
UR - http://www.scopus.com/inward/record.url?scp=0030999378&partnerID=8YFLogxK
U2 - 10.1023/A:1025653812535
DO - 10.1023/A:1025653812535
M3 - Article
C2 - 9210791
AN - SCOPUS:0030999378
SN - 0001-8244
VL - 27
SP - 201
EP - 210
JO - Behavior genetics
JF - Behavior genetics
IS - 3
ER -