Mapping epitopes for 20 monoclonal antibodies to CR1

M. Nickells, R. Hauhart, M. Krych, V. Bala Subramanian, K. Geoghegan-Barek, H. C. Marsh, J. P. Atkinson

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Abstract

Complement receptor type one (CR1; CD35) binds and processes C3b and C4b opsonized immune complexes and regulates complement activation. We have characterized the epitopes of 13 previously reported and seven new MoAbs to human CR1. The MoAbs formed seven groups based on their reactivity with a panel of deletion forms of CR1. Seventeen of the MoAbs reacted with CR1 at more than one site, a consequence of its repetitive sequence. All five of the MoAbs recognizing epitopes in the nearly identical repeats 3, 10, and 17, as well as one MoAb which reacted with repeats 8 or 1/2 of 9 and 15 or 1/2 of 16, blocked cofactor activity for C3b. Knowledge of the repeats bearing the epitopes for these MoAbs should facilitate the further characterization of CR1.

Original languageEnglish
Pages (from-to)27-33
Number of pages7
JournalClinical and Experimental Immunology
Volume112
Issue number1
DOIs
StatePublished - Apr 20 1998

Keywords

  • CD35
  • Complement control
  • Immune adherence
  • Protein repeats

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    Nickells, M., Hauhart, R., Krych, M., Bala Subramanian, V., Geoghegan-Barek, K., Marsh, H. C., & Atkinson, J. P. (1998). Mapping epitopes for 20 monoclonal antibodies to CR1. Clinical and Experimental Immunology, 112(1), 27-33. https://doi.org/10.1046/j.1365-2249.1998.00549.x