Mapping epitopes for 20 monoclonal antibodies to CR1

M. Nickells; R. Hauhart; M. Krych; V. Bala Subramanian; K. Geoghegan-Barek; H. C. Marsh; J. P. Atkinson

doi:10.1046/j.1365-2249.1998.00549.x

Mapping epitopes for 20 monoclonal antibodies to CR1

M. Nickells, R. Hauhart, M. Krych, V. Bala Subramanian, K. Geoghegan-Barek, H. C. Marsh, J. P. Atkinson

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Abstract

Complement receptor type one (CR1; CD35) binds and processes C3b and C4b opsonized immune complexes and regulates complement activation. We have characterized the epitopes of 13 previously reported and seven new MoAbs to human CR1. The MoAbs formed seven groups based on their reactivity with a panel of deletion forms of CR1. Seventeen of the MoAbs reacted with CR1 at more than one site, a consequence of its repetitive sequence. All five of the MoAbs recognizing epitopes in the nearly identical repeats 3, 10, and 17, as well as one MoAb which reacted with repeats 8 or 1/2 of 9 and 15 or 1/2 of 16, blocked cofactor activity for C3b. Knowledge of the repeats bearing the epitopes for these MoAbs should facilitate the further characterization of CR1.

Original language	English
Pages (from-to)	27-33
Number of pages	7
Journal	Clinical and Experimental Immunology
Volume	112
Issue number	1
DOIs	https://doi.org/10.1046/j.1365-2249.1998.00549.x
State	Published - 1998

Keywords

CD35
Complement control
Immune adherence
Protein repeats

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10.1046/j.1365-2249.1998.00549.x

Cite this

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title = "Mapping epitopes for 20 monoclonal antibodies to CR1",

abstract = "Complement receptor type one (CR1; CD35) binds and processes C3b and C4b opsonized immune complexes and regulates complement activation. We have characterized the epitopes of 13 previously reported and seven new MoAbs to human CR1. The MoAbs formed seven groups based on their reactivity with a panel of deletion forms of CR1. Seventeen of the MoAbs reacted with CR1 at more than one site, a consequence of its repetitive sequence. All five of the MoAbs recognizing epitopes in the nearly identical repeats 3, 10, and 17, as well as one MoAb which reacted with repeats 8 or 1/2 of 9 and 15 or 1/2 of 16, blocked cofactor activity for C3b. Knowledge of the repeats bearing the epitopes for these MoAbs should facilitate the further characterization of CR1.",

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T1 - Mapping epitopes for 20 monoclonal antibodies to CR1

AU - Nickells, M.

AU - Hauhart, R.

AU - Krych, M.

AU - Bala Subramanian, V.

AU - Geoghegan-Barek, K.

AU - Marsh, H. C.

AU - Atkinson, J. P.

PY - 1998

Y1 - 1998

N2 - Complement receptor type one (CR1; CD35) binds and processes C3b and C4b opsonized immune complexes and regulates complement activation. We have characterized the epitopes of 13 previously reported and seven new MoAbs to human CR1. The MoAbs formed seven groups based on their reactivity with a panel of deletion forms of CR1. Seventeen of the MoAbs reacted with CR1 at more than one site, a consequence of its repetitive sequence. All five of the MoAbs recognizing epitopes in the nearly identical repeats 3, 10, and 17, as well as one MoAb which reacted with repeats 8 or 1/2 of 9 and 15 or 1/2 of 16, blocked cofactor activity for C3b. Knowledge of the repeats bearing the epitopes for these MoAbs should facilitate the further characterization of CR1.

AB - Complement receptor type one (CR1; CD35) binds and processes C3b and C4b opsonized immune complexes and regulates complement activation. We have characterized the epitopes of 13 previously reported and seven new MoAbs to human CR1. The MoAbs formed seven groups based on their reactivity with a panel of deletion forms of CR1. Seventeen of the MoAbs reacted with CR1 at more than one site, a consequence of its repetitive sequence. All five of the MoAbs recognizing epitopes in the nearly identical repeats 3, 10, and 17, as well as one MoAb which reacted with repeats 8 or 1/2 of 9 and 15 or 1/2 of 16, blocked cofactor activity for C3b. Knowledge of the repeats bearing the epitopes for these MoAbs should facilitate the further characterization of CR1.

KW - CD35

KW - Complement control

KW - Immune adherence

KW - Protein repeats

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Mapping epitopes for 20 monoclonal antibodies to CR1

Abstract

Keywords

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