TY - JOUR
T1 - Mapping an endometrial cancer tumor suppressor gene at 10q25 and development of a bacterial clone contig for the consensus deletion interval
AU - Peiffer-Schneider, Stacia
AU - Noonan, Ferrin C.
AU - Mutch, David G.
AU - Simpkins, Sally B.
AU - Herzog, T.
AU - Rader, Janet
AU - Elbendary, Alaa
AU - Gersell, Deborah J.
AU - Call, Katherine
AU - Goodfellow, Paul J.
N1 - Funding Information:
This work was supported by a research grant (RPG 96-042-MGO) from the American Cancer Society. The authors gratefully acknowledge Dr. J. Mao’s contributions to mapping chromosome 10 sequences. We also thank Jeannette Apperson for her assistance in manuscript preparation.
PY - 1998/8/15
Y1 - 1998/8/15
N2 - Frequent loss of chromosome 10q sequences in endometrial cancers suggests the involvement of a tumor suppressor gene. Previous loss-of- heterozygosity (LOH) studies have pointed to the 10q25-q26 region as the likely site of a tumor suppressor involved in endometrial tumorigenesis (S. L. Peiffer et al., 1995, Cancer Res. 55: 1922-1926; S. Nagase et al., 1996, Br. J. Cancer 74: 1979-1983; 1997, Cancer Res. 57: 1630-1633). In an attempt to define further the localization of a tumor suppressor gene at 10q25, we screened a panel of 123 endometrioid adenocarcinomas for loss of heterozygosity of 10q25.3 sequences. Forty-three (35%) revealed LOH at one or more loci. The observed patterns of allelic loss define a minimum consensus region of deletion between D10S221 and D10S610. A sequence-ready bacterial clone contig and a long-range restriction map for a 1-Mb interval spanning the deletion region were developed as the first step in experiments directed toward the discovery the 10q25 tumor suppressor.
AB - Frequent loss of chromosome 10q sequences in endometrial cancers suggests the involvement of a tumor suppressor gene. Previous loss-of- heterozygosity (LOH) studies have pointed to the 10q25-q26 region as the likely site of a tumor suppressor involved in endometrial tumorigenesis (S. L. Peiffer et al., 1995, Cancer Res. 55: 1922-1926; S. Nagase et al., 1996, Br. J. Cancer 74: 1979-1983; 1997, Cancer Res. 57: 1630-1633). In an attempt to define further the localization of a tumor suppressor gene at 10q25, we screened a panel of 123 endometrioid adenocarcinomas for loss of heterozygosity of 10q25.3 sequences. Forty-three (35%) revealed LOH at one or more loci. The observed patterns of allelic loss define a minimum consensus region of deletion between D10S221 and D10S610. A sequence-ready bacterial clone contig and a long-range restriction map for a 1-Mb interval spanning the deletion region were developed as the first step in experiments directed toward the discovery the 10q25 tumor suppressor.
UR - http://www.scopus.com/inward/record.url?scp=0032529036&partnerID=8YFLogxK
U2 - 10.1006/geno.1998.5399
DO - 10.1006/geno.1998.5399
M3 - Article
C2 - 9740666
AN - SCOPUS:0032529036
SN - 0888-7543
VL - 52
SP - 9
EP - 16
JO - Genomics
JF - Genomics
IS - 1
ER -