Mapping an endometrial cancer tumor suppressor gene at 10q25 and development of a bacterial clone contig for the consensus deletion interval

Stacia Peiffer-Schneider, Ferrin C. Noonan, David G. Mutch, Sally B. Simpkins, T. Herzog, Janet Rader, Alaa Elbendary, Deborah J. Gersell, Katherine Call, Paul J. Goodfellow

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Frequent loss of chromosome 10q sequences in endometrial cancers suggests the involvement of a tumor suppressor gene. Previous loss-of- heterozygosity (LOH) studies have pointed to the 10q25-q26 region as the likely site of a tumor suppressor involved in endometrial tumorigenesis (S. L. Peiffer et al., 1995, Cancer Res. 55: 1922-1926; S. Nagase et al., 1996, Br. J. Cancer 74: 1979-1983; 1997, Cancer Res. 57: 1630-1633). In an attempt to define further the localization of a tumor suppressor gene at 10q25, we screened a panel of 123 endometrioid adenocarcinomas for loss of heterozygosity of 10q25.3 sequences. Forty-three (35%) revealed LOH at one or more loci. The observed patterns of allelic loss define a minimum consensus region of deletion between D10S221 and D10S610. A sequence-ready bacterial clone contig and a long-range restriction map for a 1-Mb interval spanning the deletion region were developed as the first step in experiments directed toward the discovery the 10q25 tumor suppressor.

Original languageEnglish
Pages (from-to)9-16
Number of pages8
JournalGenomics
Volume52
Issue number1
DOIs
StatePublished - Aug 15 1998

Fingerprint

Dive into the research topics of 'Mapping an endometrial cancer tumor suppressor gene at 10q25 and development of a bacterial clone contig for the consensus deletion interval'. Together they form a unique fingerprint.

Cite this