Mannose receptor mediated uptake of antigens strongly enhances HLA-class II restricted antigen presentation by cultured dendritic cells

M. C.Agnes A. Tan, A. Mieke Mommaas, Jan Wouter Drijfhout, Reina Jordens, Jos J.M. Onderwater, Desiree Verwoerd, Aat A. Mulder, Annette N. Van Der Heiden, Tom H.M. Ottenhoff, Marina Cella, Abraham Tulp, Jacques J. Neefjes, Frits Koning

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Dendritic cells (DCs) use macropinocytosis and mannose receptor mediated endocytosis for the uptake of exogenous antigens. Here we show that the endocytosis of the mannose receptor and mannosylated antigen is distinct from that of a non-mannosylated antigen. Shortly after internalization, however, both mannosylated and non-mannosylated antigen are found in an MHC like compartment. The mannose receptor itself does not reach this compartment, and probably releases its ligand in an earlier endosomal structure. Finally, we found that mannosylation of peptides strongly enhanced their potency to stimulate HLA class II-restricted peptide-specific T cell clones. Our results indicate that mannosylation of antigen leads to selective targeting and subsequent superior presentation by DCs which may be useful for vaccine design.

Original languageEnglish
Pages (from-to)171-174
Number of pages4
JournalAdvances in Experimental Medicine and Biology
Volume417
DOIs
StatePublished - Jan 1 1997
Externally publishedYes

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