TY - JOUR
T1 - Mannose receptor mediated uptake of antigens strongly enhances HLA-class II restricted antigen presentation by cultured dendritic cells
AU - Tan, M. C.Agnes A.
AU - Mommaas, A. Mieke
AU - Drijfhout, Jan Wouter
AU - Jordens, Reina
AU - Onderwater, Jos J.M.
AU - Verwoerd, Desiree
AU - Mulder, Aat A.
AU - Van Der Heiden, Annette N.
AU - Ottenhoff, Tom H.M.
AU - Cella, Marina
AU - Tulp, Abraham
AU - Neefjes, Jacques J.
AU - Koning, Frits
PY - 1997
Y1 - 1997
N2 - Dendritic cells (DCs) use macropinocytosis and mannose receptor mediated endocytosis for the uptake of exogenous antigens. Here we show that the endocytosis of the mannose receptor and mannosylated antigen is distinct from that of a non-mannosylated antigen. Shortly after internalization, however, both mannosylated and non-mannosylated antigen are found in an MHC like compartment. The mannose receptor itself does not reach this compartment, and probably releases its ligand in an earlier endosomal structure. Finally, we found that mannosylation of peptides strongly enhanced their potency to stimulate HLA class II-restricted peptide-specific T cell clones. Our results indicate that mannosylation of antigen leads to selective targeting and subsequent superior presentation by DCs which may be useful for vaccine design.
AB - Dendritic cells (DCs) use macropinocytosis and mannose receptor mediated endocytosis for the uptake of exogenous antigens. Here we show that the endocytosis of the mannose receptor and mannosylated antigen is distinct from that of a non-mannosylated antigen. Shortly after internalization, however, both mannosylated and non-mannosylated antigen are found in an MHC like compartment. The mannose receptor itself does not reach this compartment, and probably releases its ligand in an earlier endosomal structure. Finally, we found that mannosylation of peptides strongly enhanced their potency to stimulate HLA class II-restricted peptide-specific T cell clones. Our results indicate that mannosylation of antigen leads to selective targeting and subsequent superior presentation by DCs which may be useful for vaccine design.
UR - http://www.scopus.com/inward/record.url?scp=0030766219&partnerID=8YFLogxK
U2 - 10.1007/978-1-4757-9966-8_28
DO - 10.1007/978-1-4757-9966-8_28
M3 - Article
C2 - 9286356
AN - SCOPUS:0030766219
SN - 0065-2598
VL - 417
SP - 171
EP - 174
JO - Advances in Experimental Medicine and Biology
JF - Advances in Experimental Medicine and Biology
ER -