TY - JOUR
T1 - Mannose receptor mediated antigen uptake and presentation in human dendritic cells
AU - Engering, Anneke J.
AU - Cella, Marina
AU - Fluitsma, Donna M.
AU - Hoefsmit, Elisabeth C.M.
AU - Lanzavecchia, Antonio
AU - Pieters, Jean
PY - 1997
Y1 - 1997
N2 - In an immature state, dendritic cells (DC) can capture antigen via at least two mechanisms. First, DC use macropinocytosis for continuous uptake of large amounts of soluble antigens. Second, they express high levels of mannose receptor that can mediate internalization of glycosylated ligands. We found that dendritic cells can present mannosylated antigen 100-1000 fold more efficiently than non-mannosylated antigen. Immunocytochemistry as well as subcellular fractionation demonstrated that the mannose receptor and MHC class II molecules were located in distinct subcellular compartments. These results demonstrate that the mannose receptor endows DC with a high capacity to present glycosylated antigens at very low concentrations.
AB - In an immature state, dendritic cells (DC) can capture antigen via at least two mechanisms. First, DC use macropinocytosis for continuous uptake of large amounts of soluble antigens. Second, they express high levels of mannose receptor that can mediate internalization of glycosylated ligands. We found that dendritic cells can present mannosylated antigen 100-1000 fold more efficiently than non-mannosylated antigen. Immunocytochemistry as well as subcellular fractionation demonstrated that the mannose receptor and MHC class II molecules were located in distinct subcellular compartments. These results demonstrate that the mannose receptor endows DC with a high capacity to present glycosylated antigens at very low concentrations.
UR - http://www.scopus.com/inward/record.url?scp=0030870417&partnerID=8YFLogxK
U2 - 10.1007/978-1-4757-9966-8_31
DO - 10.1007/978-1-4757-9966-8_31
M3 - Article
C2 - 9286359
AN - SCOPUS:0030870417
SN - 0065-2598
VL - 417
SP - 183
EP - 187
JO - Advances in Experimental Medicine and Biology
JF - Advances in Experimental Medicine and Biology
ER -