Mannose receptor mediated antigen uptake and presentation in human dendritic cells

Anneke J. Engering, Marina Cella, Donna M. Fluitsma, Elisabeth C.M. Hoefsmit, Antonio Lanzavecchia, Jean Pieters

Research output: Contribution to journalArticlepeer-review

98 Scopus citations

Abstract

In an immature state, dendritic cells (DC) can capture antigen via at least two mechanisms. First, DC use macropinocytosis for continuous uptake of large amounts of soluble antigens. Second, they express high levels of mannose receptor that can mediate internalization of glycosylated ligands. We found that dendritic cells can present mannosylated antigen 100-1000 fold more efficiently than non-mannosylated antigen. Immunocytochemistry as well as subcellular fractionation demonstrated that the mannose receptor and MHC class II molecules were located in distinct subcellular compartments. These results demonstrate that the mannose receptor endows DC with a high capacity to present glycosylated antigens at very low concentrations.

Original languageEnglish
Pages (from-to)183-187
Number of pages5
JournalAdvances in Experimental Medicine and Biology
Volume417
StatePublished - Oct 18 1997

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