Manganese stimulates incorporation of [3H]inositol into an agonist - Insensitive pool of phosphatidylinositol in brain membranes

Rodrigo Labarca; Aaron Janowsky; Steven M. Paul

doi:10.1016/0006-291X(85)91167-2

Manganese stimulates incorporation of [³H]inositol into an agonist - Insensitive pool of phosphatidylinositol in brain membranes

Rodrigo Labarca, Aaron Janowsky, Steven M. Paul

Washington University School of Medicine

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Abstract

In a particulate preparation from rat brain, manganese ions stimulate the incorporation of [³H]inositol into inositol phospholipids in a concentration-dependent manner. Incubation with CDP-diacylglycerol (0.5 mM) alone had no effect on the incorporation of [³H]inositol but potentiated the stimulatory effect of manganese. Despite the increase in [³H]inositol incorporation into phosphatidylinositol, the carbachol-induced accumulation of [³H]inositol-1-phosphate was unaltered in membranes preincubated with manganese but when coincubated with CDP-diacylglycerol the carbachol-induced accumulation of [³H]inositol-1-phosphate was increased. These data suggest that manganese stimulates the incorporation of [³H]inositol into an agonist-insenstive pool of phosphatidylinositol.

Original language	English
Pages (from-to)	540-547
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	132
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(85)91167-2
State	Published - Oct 30 1985

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title = "Manganese stimulates incorporation of [3H]inositol into an agonist - Insensitive pool of phosphatidylinositol in brain membranes",

abstract = "In a particulate preparation from rat brain, manganese ions stimulate the incorporation of [3H]inositol into inositol phospholipids in a concentration-dependent manner. Incubation with CDP-diacylglycerol (0.5 mM) alone had no effect on the incorporation of [3H]inositol but potentiated the stimulatory effect of manganese. Despite the increase in [3H]inositol incorporation into phosphatidylinositol, the carbachol-induced accumulation of [3H]inositol-1-phosphate was unaltered in membranes preincubated with manganese but when coincubated with CDP-diacylglycerol the carbachol-induced accumulation of [3H]inositol-1-phosphate was increased. These data suggest that manganese stimulates the incorporation of [3H]inositol into an agonist-insenstive pool of phosphatidylinositol.",

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N2 - In a particulate preparation from rat brain, manganese ions stimulate the incorporation of [3H]inositol into inositol phospholipids in a concentration-dependent manner. Incubation with CDP-diacylglycerol (0.5 mM) alone had no effect on the incorporation of [3H]inositol but potentiated the stimulatory effect of manganese. Despite the increase in [3H]inositol incorporation into phosphatidylinositol, the carbachol-induced accumulation of [3H]inositol-1-phosphate was unaltered in membranes preincubated with manganese but when coincubated with CDP-diacylglycerol the carbachol-induced accumulation of [3H]inositol-1-phosphate was increased. These data suggest that manganese stimulates the incorporation of [3H]inositol into an agonist-insenstive pool of phosphatidylinositol.

AB - In a particulate preparation from rat brain, manganese ions stimulate the incorporation of [3H]inositol into inositol phospholipids in a concentration-dependent manner. Incubation with CDP-diacylglycerol (0.5 mM) alone had no effect on the incorporation of [3H]inositol but potentiated the stimulatory effect of manganese. Despite the increase in [3H]inositol incorporation into phosphatidylinositol, the carbachol-induced accumulation of [3H]inositol-1-phosphate was unaltered in membranes preincubated with manganese but when coincubated with CDP-diacylglycerol the carbachol-induced accumulation of [3H]inositol-1-phosphate was increased. These data suggest that manganese stimulates the incorporation of [3H]inositol into an agonist-insenstive pool of phosphatidylinositol.

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Manganese stimulates incorporation of [³H]inositol into an agonist - Insensitive pool of phosphatidylinositol in brain membranes

Abstract

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