Manganese stimulates incorporation of [3H]inositol into an agonist - Insensitive pool of phosphatidylinositol in brain membranes

Rodrigo Labarca, Aaron Janowsky, Steven M. Paul

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

In a particulate preparation from rat brain, manganese ions stimulate the incorporation of [3H]inositol into inositol phospholipids in a concentration-dependent manner. Incubation with CDP-diacylglycerol (0.5 mM) alone had no effect on the incorporation of [3H]inositol but potentiated the stimulatory effect of manganese. Despite the increase in [3H]inositol incorporation into phosphatidylinositol, the carbachol-induced accumulation of [3H]inositol-1-phosphate was unaltered in membranes preincubated with manganese but when coincubated with CDP-diacylglycerol the carbachol-induced accumulation of [3H]inositol-1-phosphate was increased. These data suggest that manganese stimulates the incorporation of [3H]inositol into an agonist-insenstive pool of phosphatidylinositol.

Original languageEnglish
Pages (from-to)540-547
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume132
Issue number2
DOIs
StatePublished - Oct 30 1985
Externally publishedYes

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