MALT1 Protease Activation Triggers Acute Disruption of Endothelial Barrier Integrity via CYLD Cleavage

Linda R. Klei, Dong Hu, Robert Panek, Danielle N. Alfano, Rachel E. Bridwell, Kelly M. Bailey, Katherine I. Oravecz-Wilson, Vincent J. Concel, Emily M. Hess, Matthew Van Beek, Phillip C. Delekta, Shufang Gu, Simon C. Watkins, Adrian T. Ting, Peter J. Gough, Kevin P. Foley, John Bertin, Linda M. McAllister-Lucas, Peter C. Lucas

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Microvascular endothelial cells maintain a tight barrier to prevent passage of plasma and circulating immune cells into the extravascular tissue compartment, yet endothelial cells respond rapidly to vasoactive substances, including thrombin, allowing transient paracellular permeability. This response is a cornerstone of acute inflammation, but the mechanisms responsible are still incompletely understood. Here, we demonstrate that thrombin triggers MALT1 to proteolytically cleave cylindromatosis (CYLD). Fragmentation of CYLD results in microtubule disruption and a cascade of events leading to endothelial cell retraction and an acute permeability response. This finding reveals an unexpected role for the MALT1 protease, which previously has been viewed mostly as a driver of pro-inflammatory NF-κB signaling in lymphocytes. Thus, MALT1 not only promotes immune cell activation but also acutely regulates endothelial cell biology, actions that together facilitate tissue inflammation. Pharmacologic inhibition of MALT1 may therefore have synergistic impact by targeting multiple disparate steps in the overall inflammatory response.

Original languageEnglish
Pages (from-to)221-232
Number of pages12
JournalCell Reports
Volume17
Issue number1
DOIs
StatePublished - Sep 27 2016
Externally publishedYes

Keywords

  • Bcl10
  • CARD10
  • CARMA3
  • CYLD
  • G protein-coupled receptor (GPCR)
  • MALT1 protease
  • NF-κB
  • Protease activated receptor-1 (PAR1)
  • endothelial permeability
  • thrombin

Fingerprint Dive into the research topics of 'MALT1 Protease Activation Triggers Acute Disruption of Endothelial Barrier Integrity via CYLD Cleavage'. Together they form a unique fingerprint.

  • Cite this

    Klei, L. R., Hu, D., Panek, R., Alfano, D. N., Bridwell, R. E., Bailey, K. M., Oravecz-Wilson, K. I., Concel, V. J., Hess, E. M., Van Beek, M., Delekta, P. C., Gu, S., Watkins, S. C., Ting, A. T., Gough, P. J., Foley, K. P., Bertin, J., McAllister-Lucas, L. M., & Lucas, P. C. (2016). MALT1 Protease Activation Triggers Acute Disruption of Endothelial Barrier Integrity via CYLD Cleavage. Cell Reports, 17(1), 221-232. https://doi.org/10.1016/j.celrep.2016.08.080