TY - JOUR
T1 - Male-to-female sex reversal in mice lacking fibroblast growth factor 9
AU - Colvin, Jennifer S.
AU - Green, Rebecca P.
AU - Schmahl, Jennifer
AU - Capel, Blanche
AU - Ornitz, David M.
N1 - Funding Information:
We thank A. McMahon and P. Koopman for in situ probes; N. Josso, A. Parlow (NIDDK National Hormone and Pituitary Program), and L. Muglia for antibodies; R. Hammer and S. Maika for SM-1 ES cells; and K. Thomas for TK and Pol2-NEO selection cassettes. We are grateful for assistance from: E. Taylor, M. Scott, A. Johnson, and B. Coleman (histology); E. Spinaio, X. Hua, L. Li, and H. Walker (animal husbandry); L. LaRose and M. Levy (TEM); J. Waggoner (figures); and O. M. Colvin, L. Muglia, I. Boime, and R. Kopan (manuscript review). The SSEA-1 antibody, developed by D. Solter, was obtained from the Developmental Studies Hybridoma Bank (NICHD) at the University of Iowa. This work was funded by grants from: Monsanto/Searle/Pharmacia Inc. (D. M. O.), the Genentech Foundation for Growth and Development (R. P. G.), training grant 5-T32-GM07200-25 (J. S. C.), NIH grants HD33688 (R. P. G.), CA60673 (D. M. O.), HD35692 (D. M. O.), GM56757 (B. C.), and NSF grant IBN-9905685 (B. C.).
PY - 2001/3/23
Y1 - 2001/3/23
N2 - Fgfs direct embryogenesis of several organs, including the lung, limb, and anterior pituitary. Here we report male-to-female sex reversal in mice lacking Fibroblast growth factor 9 (Fgf9), demonstrating a novel role for FGF signaling in testicular embryogenesis. Fgf9-/- mice also exhibit lung hypoplasia and die at birth. Reproductive system phenotypes range from testicular hypoplasia to complete sex reversal, with most Fgf9-/- XY reproductive systems appearing grossly female at birth. Fgf9 appears to act downstream of Sry to stimulate mesenchymal proliferation, mesonephric cell migration, and Sertoli cell differentiation in the embryonic testis. While Sry is found only in some mammals, Fgfs are highly conserved. Thus, Fgfs may function in sex determination and reproductive system development in many species.
AB - Fgfs direct embryogenesis of several organs, including the lung, limb, and anterior pituitary. Here we report male-to-female sex reversal in mice lacking Fibroblast growth factor 9 (Fgf9), demonstrating a novel role for FGF signaling in testicular embryogenesis. Fgf9-/- mice also exhibit lung hypoplasia and die at birth. Reproductive system phenotypes range from testicular hypoplasia to complete sex reversal, with most Fgf9-/- XY reproductive systems appearing grossly female at birth. Fgf9 appears to act downstream of Sry to stimulate mesenchymal proliferation, mesonephric cell migration, and Sertoli cell differentiation in the embryonic testis. While Sry is found only in some mammals, Fgfs are highly conserved. Thus, Fgfs may function in sex determination and reproductive system development in many species.
UR - https://www.scopus.com/pages/publications/0035937405
U2 - 10.1016/S0092-8674(01)00284-7
DO - 10.1016/S0092-8674(01)00284-7
M3 - Article
C2 - 11290325
AN - SCOPUS:0035937405
SN - 0092-8674
VL - 104
SP - 875
EP - 889
JO - Cell
JF - Cell
IS - 6
ER -