Major role of collagen IIB in the elevation of total type II procollagen messenger RNA in the hypertrophic phase of experimental osteoarthritis

John R. Matyas, Mark E. Adams, Dinqqiu Huang, Linda J. Sandell

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Objective. To define the relative contributions of procollagen IIA and IIB messenger RNA (mRNA) in the 8-fold elevation of total (IIA + IIB) type II procollagen mRNA in cartilage from joints with early experimental osteoarthritis (OA). Methods. Unilateral cruciate ligament transection was performed on skeletally mature animals. Total RNA was extracted from articular cartilage from control joints and from joints with experimental OA. Northern blot hybridization with oligoprobes was used to distinguish between types IIA and IIb mRNA. Results. Levels of IIA mRNA were similar in control and experimental cartilage. Type IIB mRNA accounted for the increase in total type II mRNA expression in OA joints. Conclusion. Although OA chondrocytes adapt to making immature forms of aggrecan proteoglycan, they do not revert to the prechondrocyte stage characterized by IIA mRNA expression.

Original languageEnglish
Pages (from-to)1046-1049
Number of pages4
JournalArthritis and rheumatism
Volume40
Issue number6
DOIs
StatePublished - 1997

Fingerprint

Dive into the research topics of 'Major role of collagen IIB in the elevation of total type II procollagen messenger RNA in the hypertrophic phase of experimental osteoarthritis'. Together they form a unique fingerprint.

Cite this