TY - JOUR
T1 - Maf b lineage tracing to distinguish macrophages from other immune lineages reveals dual identity of langerhans cells
AU - Wu, Xiaodi
AU - Briseño, Carlos G.
AU - Durai, Vivek
AU - Albring, Jorn C.
AU - Haldar, Malay
AU - Bagadia, Prachi
AU - Kim, Ki Wook
AU - Randolph, Gwendalyn J.
AU - Murphy, Theresa L.
AU - Murphy, Kenneth M.
PY - 2016/11/14
Y1 - 2016/11/14
N2 - Current systems for conditional gene deletion within mouse macrophage lineages are limited by ectopic activity or low efficiency. In this study, we generated a Mafb-driven Cre strain to determine whether any dendritic cells (DCs) identified by Zbtb46-GFP expression originate from a Mafb-expressing population. Lineage tracing distinguished macrophages from classical DCs, neutrophils, and B cells in all organs examined. At steady state, Langerhans cells (LCs) were lineage traced but also expressed Zbtb46-GFP, a phenotype not observed in any other population. After exposure to house dust mite antigen, Zbtb46-negative CD64+ inflammatory cells infiltrating the lung were substantially lineage traced, but Zbtb46-positive CD64- cells were not. These results provide new evidence for the unique identity of LCs and challenge the notion that some inflammatory cells are a population of monocyte-derived DCs.
AB - Current systems for conditional gene deletion within mouse macrophage lineages are limited by ectopic activity or low efficiency. In this study, we generated a Mafb-driven Cre strain to determine whether any dendritic cells (DCs) identified by Zbtb46-GFP expression originate from a Mafb-expressing population. Lineage tracing distinguished macrophages from classical DCs, neutrophils, and B cells in all organs examined. At steady state, Langerhans cells (LCs) were lineage traced but also expressed Zbtb46-GFP, a phenotype not observed in any other population. After exposure to house dust mite antigen, Zbtb46-negative CD64+ inflammatory cells infiltrating the lung were substantially lineage traced, but Zbtb46-positive CD64- cells were not. These results provide new evidence for the unique identity of LCs and challenge the notion that some inflammatory cells are a population of monocyte-derived DCs.
UR - http://www.scopus.com/inward/record.url?scp=84999752022&partnerID=8YFLogxK
U2 - 10.1084/jem.20160600
DO - 10.1084/jem.20160600
M3 - Article
C2 - 27810926
AN - SCOPUS:84999752022
SN - 0022-1007
VL - 213
SP - 2553
EP - 2565
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
IS - 12
ER -