Maf b lineage tracing to distinguish macrophages from other immune lineages reveals dual identity of langerhans cells

Xiaodi Wu, Carlos G. Briseño, Vivek Durai, Jorn C. Albring, Malay Haldar, Prachi Bagadia, Ki Wook Kim, Gwendalyn J. Randolph, Theresa L. Murphy, Kenneth M. Murphy

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

Current systems for conditional gene deletion within mouse macrophage lineages are limited by ectopic activity or low efficiency. In this study, we generated a Mafb-driven Cre strain to determine whether any dendritic cells (DCs) identified by Zbtb46-GFP expression originate from a Mafb-expressing population. Lineage tracing distinguished macrophages from classical DCs, neutrophils, and B cells in all organs examined. At steady state, Langerhans cells (LCs) were lineage traced but also expressed Zbtb46-GFP, a phenotype not observed in any other population. After exposure to house dust mite antigen, Zbtb46-negative CD64+ inflammatory cells infiltrating the lung were substantially lineage traced, but Zbtb46-positive CD64- cells were not. These results provide new evidence for the unique identity of LCs and challenge the notion that some inflammatory cells are a population of monocyte-derived DCs.

Original languageEnglish
Pages (from-to)2553-2565
Number of pages13
JournalJournal of Experimental Medicine
Volume213
Issue number12
DOIs
StatePublished - Nov 14 2016

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