Macrophage activation: Synergism between hybridoma MAF and poly(I). Poly(C) delivered by liposomes

C. Pidgeon, R. D. Schreiber, R. M. Schultz

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19 Scopus citations

Abstract

High concentrations of a murine T cell hybridoma culture supernatant containing macrophage-activating factor (MAF) rendered resident mouse peritoneal macrophages cytotoxic for P815 mastocytoma cells. The capacity of the hybridoma-derived MAF [MAF(H)] to induce tumoricidal activity increased 103 to 104-fold when the lymphokine was excapsulated into liposomes. Combinations of MAF(H) and poly(I) · poly(C) acted synergistically to render macrophages potency cytotoxic. Subthreshold (nonactivating) concentrations of free or liposome-encapsulated MAF(H) increased the potency of free poly(I) · poly(C) and liposome encapsulated poly(I) · poly(C). Either as free agent or encapsulated in liposomes, single-stranded poly(I) or poly(C) did not activate macrophages in the presence or absence of MAF(H). Double-stranded poly(I) · poly(C) was thus required for macrophage activation and synergism with MAF(H).

Original languageEnglish
Pages (from-to)311-314
Number of pages4
JournalJournal of Immunology
Volume131
Issue number1
StatePublished - Aug 25 1983

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