Macrophage activation increases the invasive properties of hepatoma cells by destabilization of the adherens junction

Chieh Yu Lin, Chien Jung Lin, Kuo Hsing Chen, Jiann Chun Wu, Shih Horng Huang, Seu Mei Wang

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

Tumor-associated macrophages play an important role in tumor progression, but whether they exert a tumor-progressive effect remains controversial. Here, we demonstrated that activated macrophage-conditioned medium (AMCM) obtained from RAW macrophages (RAW/AMCM) induced epithelial-mesenchymal transition (EMT) and stimulated the migratory and invasive activities of HepG2 cells, whereas control conditioned media had no effect. Epithelial-cadherin (E-cadherin) and β-catenin staining patterns were altered at the adherens junctions by RAW/AMCM treatment, with an approximately 50% decrease in E-cadherin and β-catenin in the cell membrane. Importantly, levels of β-catenin-associated E-cadherin were also decreased. Following RAW/AMCM treatment, enhanced activation of c-Src was seen prior to increased tyrosine phosphorylation of β-catenin, and this led to the destabilization of adherens junctions. Pretreatment of HepG2 cells with the Src kinase inhibitor, PP2, completely abolished the effects of RAW/AMCM on the EMT, migration, invasion, and expression and association of E-cadherin and β-catenin. AMCMs obtained from human THP-1 monocytes and mouse peritoneal macrophages also caused disassembly of the adherens junctions and migration of HepG2 cells. Furthermore, inhibition of the epidermal growth factor receptor (EGFR) with gefitinib partially prevented the downregulation of E-cadherin and β-catenin at the adherens junctions and migration behavior induced by RAW/AMCM. Our results suggest that activated macrophages have a tumor-progressive effect on HepG2 cells which involves the c-Src- and EGFR-dependent signaling cascades.

Original languageEnglish
Pages (from-to)3042-3050
Number of pages9
JournalFEBS Letters
Volume580
Issue number13
DOIs
StatePublished - May 29 2006
Externally publishedYes

Keywords

  • Epidermal growth factor receptor
  • Epithelial-cadherin
  • Macrophages
  • Metastasis
  • Tyrosine phosphorylation of β-catenin
  • c-Src kinase
  • β-Catenin

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