TY - JOUR
T1 - Lymphatic cannulation for lymph sampling and molecular delivery
AU - Zawieja, David C.
AU - Thangaswamy, Sangeetha
AU - Wang, Wei
AU - Furtado, Raquel
AU - Clement, Cristina C.
AU - Papadopoulos, Zachary
AU - Vigano, Marco
AU - Bridenbaugh, Eric A.
AU - Zolla, Lello
AU - Gashev, Anatoliy A.
AU - Kipnis, Jonathan
AU - Lauvau, Gregoire
AU - Santambrogio, Laura
N1 - Funding Information:
*Department of Medical Physiology, Texas A&M Health Science Center, Temple, TX 76504; †Department of Pathology, Albert Einstein College of Medicine, Mon-tefiore Medical Center, New York, NY 10461; ‡Department of Microbiology and Immunology, Albert Einstein College of Medicine, Montefiore Medical Center, New York, NY 10461; xCenter for Brain Immunology and Glia, School of Medicine, University of Virginia, Charlottesville, VA 22908; {Department of Neuroscience, School of Medicine, University of Virginia, Charlottesville, VA 22908; ‖Orthopaedic Biotechnology Lab, Galeazzi Orthopaedic Institute for Care and Scientific Research, 20161 Milan, Italy; and #Department of Agricultural and Forest Sciences, University La Tuscia, 01100 Viterbo, Italy
Funding Information:
This work was supported by National Institutes of Health Grants AG045223 and AI137198 (to L.S.) and 1U01HL123420 (to D.C.Z. and A.A.G.).
Publisher Copyright:
Copyright © 2019 by The American Association of Immunologists, Inc.
PY - 2019/10/15
Y1 - 2019/10/15
N2 - Unlike the blood, the interstitial fluid and the deriving lymph are directly bathing the cellular layer of each organ. As such, composition analysis of the lymphatic fluid can provide more precise biochemical and cellular information on an organ's health and be a valuable resource for biomarker discovery. In this study, we describe a protocol for cannulation of mouse and rat lymphatic collectors that is suitable for the following: the "omic" sampling of pre- and postnodal lymph, collected from different anatomical districts; the phenotyping of immune cells circulating between parenchymal organs and draining lymph nodes; injection of known amounts of molecules for quantitative immunological studies of nodal trafficking and/or clearance; and monitoring an organ's biochemical omic changes in pathological conditions. Our data indicate that probing the lymphatic fluid can provide an accurate snapshot of an organ's physiology/pathology, making it an ideal target for liquid biopsy.
AB - Unlike the blood, the interstitial fluid and the deriving lymph are directly bathing the cellular layer of each organ. As such, composition analysis of the lymphatic fluid can provide more precise biochemical and cellular information on an organ's health and be a valuable resource for biomarker discovery. In this study, we describe a protocol for cannulation of mouse and rat lymphatic collectors that is suitable for the following: the "omic" sampling of pre- and postnodal lymph, collected from different anatomical districts; the phenotyping of immune cells circulating between parenchymal organs and draining lymph nodes; injection of known amounts of molecules for quantitative immunological studies of nodal trafficking and/or clearance; and monitoring an organ's biochemical omic changes in pathological conditions. Our data indicate that probing the lymphatic fluid can provide an accurate snapshot of an organ's physiology/pathology, making it an ideal target for liquid biopsy.
UR - http://www.scopus.com/inward/record.url?scp=85072992879&partnerID=8YFLogxK
U2 - 10.4049/jimmunol.1900375
DO - 10.4049/jimmunol.1900375
M3 - Article
C2 - 31519866
AN - SCOPUS:85072992879
SN - 0022-1767
VL - 203
SP - 2339
EP - 2350
JO - Journal of Immunology
JF - Journal of Immunology
IS - 8
ER -