Lymphatic cannulation for lymph sampling and molecular delivery

David C. Zawieja, Sangeetha Thangaswamy, Wei Wang, Raquel Furtado, Cristina C. Clement, Zachary Papadopoulos, Marco Vigano, Eric A. Bridenbaugh, Lello Zolla, Anatoliy A. Gashev, Jonathan Kipnis, Gregoire Lauvau, Laura Santambrogio

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


Unlike the blood, the interstitial fluid and the deriving lymph are directly bathing the cellular layer of each organ. As such, composition analysis of the lymphatic fluid can provide more precise biochemical and cellular information on an organ's health and be a valuable resource for biomarker discovery. In this study, we describe a protocol for cannulation of mouse and rat lymphatic collectors that is suitable for the following: the "omic" sampling of pre- and postnodal lymph, collected from different anatomical districts; the phenotyping of immune cells circulating between parenchymal organs and draining lymph nodes; injection of known amounts of molecules for quantitative immunological studies of nodal trafficking and/or clearance; and monitoring an organ's biochemical omic changes in pathological conditions. Our data indicate that probing the lymphatic fluid can provide an accurate snapshot of an organ's physiology/pathology, making it an ideal target for liquid biopsy.

Original languageEnglish
Pages (from-to)2339-2350
Number of pages12
JournalJournal of Immunology
Issue number8
StatePublished - Oct 15 2019


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