Low peak bone mass and attenuated anabolic response to parathyroid hormone in mice with an osteoblast-specific deletion of connexin43

Dong Jin Chung, Charlles H.M. Castro, Marcus Watkins, Joseph P. Stains, Min Young Chung, Vera Lucia Szejnfeld, Klaus Willecke, Martin Theis, Roberto Civitelli

Research output: Contribution to journalArticlepeer-review

140 Scopus citations

Abstract

Connexin43 (Cx43) is involved in bone development, but its role in adult bone homeostasis remains unknown. To overcome the postnatal lethality of Cx43 null mutation, we generated mice with selective osteoblast ablation of Cx43, obtained using a Cx43fl allele and a 2.3-kb fragment of the αI(I) collagen promoter to drive Cre in osteoblasts (ColCre). Conditionally osteoblast-deleted ColCre;Cx43-fl mice show no malformations at birth, but develop low peak bone mass and remain osteopenic with age, exhibiting reduced bone formation and defective osteoblast function. By both radiodensitometry and histology, bone mineral content increased rapidly and progressively in adult Cx43+/ fl mice after subcutaneous injection of parathyroid hormone (PTH), an effect significantly attenuated in ColCre;Cx43-/fl mice, with Cx43-/ fl exhibiting an intermediate response. Attenuation of PTH anabolic action was associated with failure to increase mineral apposition rate in response to PTH in ColCre;CW34-/fl, despite an increased osteoblast number, suggesting a functional defect in Cx43-deficient bone-forming cells. In conclusion, lack of Cx43 in osteoblasts leads to suboptimal acquisition of peak bone mass, and hinders the bone anabolic effect of PTH. Cx43 represents a potential target for modulation of bone anabolism.

Original languageEnglish
Pages (from-to)4187-4198
Number of pages12
JournalJournal of cell science
Volume119
Issue number20
DOIs
StatePublished - Oct 15 2006

Keywords

  • Bone anabolic agents
  • Conditional gene deletion
  • Connexin43
  • Gap junctions
  • Teriparatide

Fingerprint

Dive into the research topics of 'Low peak bone mass and attenuated anabolic response to parathyroid hormone in mice with an osteoblast-specific deletion of connexin43'. Together they form a unique fingerprint.

Cite this