Low-cost disposable point-of-care devices based on porous materials for rapid detection of pathogens

P. Yager, K. Abe, C. E. Anderson, D. Baker, Y. Belousov, J. Bishop, J. R. Buser, S. A. Byrnes, G. Domingo, E. Fu, R. P. Gallagher, E. K. Heiniger, C. A. Holstein, S. Huang, P. Kauffman, E. C. Kline, P. D. Ladd, L. K. Lafleur, B. R. Lutz, W. MahoneyS. Kumar, N. Scarr, L. Stewart, E. M. Strauch, B. Toley, N. M.J. Vermeulen, X. Zhang

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Porous materials such as nitrocellulose, cellulose and glass fiber, when combined with switchable interconnections, support complex sequential chemical processes like sample-to-result chemical detection with minimal supporting equipment, and at extremely low cost. Using such two-dimensional paper networks (2DPNs), we have demonstrated user-friendly chemically-amplified sensitive detection of influenza viruses and proteins derived therefrom; we used both antibodies and capture molecules designed de novo as inexpensive tailored alternatives. By combining 2DPNs with isothermal nucleic acid amplification, we have demonstrated an instrument-free disposable platform that performs rapid sample-to-result detection of pathogens by DNA and RNA at sensitivities rivalling the best instrumented systems.

Original languageEnglish
Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PublisherChemical and Biological Microsystems Society
Pages97-100
Number of pages4
ISBN (Electronic)9780692941836
StatePublished - 2020
Event21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States
Duration: Oct 22 2017Oct 26 2017

Publication series

Name21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Conference

Conference21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
Country/TerritoryUnited States
CitySavannah
Period10/22/1710/26/17

Keywords

  • Diagnostics
  • Inexpensive
  • Microfluidics
  • Paper

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