TY - JOUR
T1 - Loss-of-susceptibility mutants of Arabidopsis thaliana reveal an essential role for elF(iso)4E during potyvirus infection
AU - Lellis, Andrew D.
AU - Kasschau, Kristin D.
AU - Whitham, Steven A.
AU - Carrington, James C.
N1 - Funding Information:
We thank Dr. Karen Browning for supplying purified eIF(iso)4E and anti-eIF(iso)4E serum and Dr. Fernando Ponz for providing the initial infectious TuMV clone. This work was supported by grants from the National Institutes of Health (AI43288 and AI27832) and the United States Department of Agriculture (NRI 2002-35319-11560).
PY - 2002
Y1 - 2002
N2 - The Arabidopsis thaliana-potyvirus system was developed to identify compatibility and incompatibility factors involved during infection and disease caused by positive-strand RNA viruses [1,2]. Several Arabidopsis mutants with increased susceptibility to Tobacco etch potyvirus (TEV) were isolated previously [1], revealing a virus-specific resistance system in the phloem [3-5]. In this study, Arabidopsis mutants with decreased susceptibility to Turnip mosaic potyvirus (TuMV) were isolated. Three independent mutants that conferred immunity to TuMV were isolated and assigned to the same complementation group. These mutants were also immune or near-immune to TEV but were susceptible to an unrelated virus. The locus associated with decreased susceptibility was named loss-of-susceptibility to potyviruses 1 (Isp1). The LSP1 locus was isolated by map-based cloning and was identified as the gene encoding translation factor elF(iso)4E, one of several known Arabidopsis isoforms that has cap binding activity, elF4E and elF(iso)4E from different plant species were shown previously to interact with the genomelinked protein (VPg) of TEV and TuMV, respectively. Models to explain the roles of elF(iso)4E during virus infection are presented.
AB - The Arabidopsis thaliana-potyvirus system was developed to identify compatibility and incompatibility factors involved during infection and disease caused by positive-strand RNA viruses [1,2]. Several Arabidopsis mutants with increased susceptibility to Tobacco etch potyvirus (TEV) were isolated previously [1], revealing a virus-specific resistance system in the phloem [3-5]. In this study, Arabidopsis mutants with decreased susceptibility to Turnip mosaic potyvirus (TuMV) were isolated. Three independent mutants that conferred immunity to TuMV were isolated and assigned to the same complementation group. These mutants were also immune or near-immune to TEV but were susceptible to an unrelated virus. The locus associated with decreased susceptibility was named loss-of-susceptibility to potyviruses 1 (Isp1). The LSP1 locus was isolated by map-based cloning and was identified as the gene encoding translation factor elF(iso)4E, one of several known Arabidopsis isoforms that has cap binding activity, elF4E and elF(iso)4E from different plant species were shown previously to interact with the genomelinked protein (VPg) of TEV and TuMV, respectively. Models to explain the roles of elF(iso)4E during virus infection are presented.
UR - https://www.scopus.com/pages/publications/0037172664
U2 - 10.1016/S0960-9822(02)00898-9
DO - 10.1016/S0960-9822(02)00898-9
M3 - Article
C2 - 12123581
AN - SCOPUS:0037172664
SN - 0960-9822
VL - 12
SP - 1046
EP - 1051
JO - Current Biology
JF - Current Biology
IS - 12
M1 - 1822
ER -