@article{40692ededd43456a96df393a2278532b,
title = "Loss of mir146b with aging contributes to inflammation and mitochondrial dysfunction in thioglycollate-elicited peritoneal macrophages",
abstract = "Macrophages undergo programmatic changes with age leading to altered cytokine polarization and immune dysfunction, shifting these critical immune cells from protective sentinels to disease promoters. The molecular mechanisms underlying macrophage inflammaging are poorly understood. Using an unbiased RNA-seq approach, we identified Mir146b as a microRNA whose expression progressively and unidirectionally declined with age in thioglycollate-elicited murine macrophages. Mir146b deficiency led to altered macrophage cytokine expression and reduced mitochondrial metabolic activity, two hallmarks of cellular aging. Single cell RNA sequencing identified patterns of altered inflammation and interferon gamma signaling in Mir146b deficient macrophages. Identification of Mir146b as a potential regulator of macrophage aging provides novel insights into immune dysfunction associated with aging.",
author = "Santeford, {Andrea C.} and Lee, {Aaron Y.} and Abdoulaye Sene and Hassman, {Lynn M.} and Sergushichev, {Alexey A.} and Ekaterina Loginicheva and Artyomov, {Maxim N.} and Ruzycki, {Phillip A.} and Apte, {Rajendra S.}",
note = "Funding Information: This work was supported by NIH grant R01 EY019287-08 (RSA), P30 EY02687 (Vision Funding Information: Fund (RSA), the Starr Foundation (RSA), and an unrestricted grant from Research to Funding Information: Core Grant), Glenn Foundation for Medical Research Award (RSA), American Funding Information: This work was supported by NIH grant R01 EY019287-08 (RSA), P30 EY02687 (Vision Core Grant), Glenn Foundation for Medical Research Award (RSA), American Federation for Aging Research Julie Martin Mid-Career Award (RSA), Carl Marshall Reeves and Mildred Almen Reeves Foundation Award (RSA), Jeffery T. Fort Innovation Fund (RSA), the Starr Foundation (RSA), and an unrestricted grant from Research to Prevent Blindness to the John F. Hardesty, MD Department of Ophthalmology and Visual Sciences at Washington University School of Medicine in Saint Louis. We would like to thank Jonathan B. Lin, MD, PhD for his assistance with statistical analysis. Publisher Copyright: {\textcopyright} 2021, eLife Sciences Publications Ltd. All rights reserved.",
year = "2021",
month = aug,
doi = "10.7554/eLife.66703",
language = "English",
volume = "10",
journal = "eLife",
issn = "2050-084X",
}