Loop-mediated isothermal amplification assay for rapid detection of common strains of Escherichia coli

Joshua Hill; Shilpa Beriwal; Ishwad Chandra; Vinod K. Paul; Aarti Kapil; Tripti Singh; Robert M. Wadowsky; Vinita Singh; Ankur Goyal; Timo Jahnukainen; James R. Johnson; Phillip I. Tarr; Abhay Vats

doi:10.1128/JCM.00152-08

Loop-mediated isothermal amplification assay for rapid detection of common strains of Escherichia coli

Joshua Hill
, Shilpa Beriwal
, Ishwad Chandra
, Vinod K. Paul
, Aarti Kapil
, Tripti Singh
, Robert M. Wadowsky
, Vinita Singh
, Ankur Goyal
, Timo Jahnukainen
, James R. Johnson
, Phillip I. Tarr
, Abhay Vats

Research output: Contribution to journal › Article › peer-review

237 Scopus citations

Abstract

We developed a highly sensitive and specific LAMP assay for Escherichia coli. It does not require DNA extraction and can detect as few as 10 copies. It detected all 36 of 36 E. coli isolates and all 22 urine samples (out of 89 samples tested) that had E. coli. This assay is rapid, low in cost, and simple to perform.

Original language	English
Pages (from-to)	2800-2804
Number of pages	5
Journal	Journal of clinical microbiology
Volume	46
Issue number	8
DOIs	https://doi.org/10.1128/JCM.00152-08
State	Published - Aug 2008

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title = "Loop-mediated isothermal amplification assay for rapid detection of common strains of Escherichia coli",

abstract = "We developed a highly sensitive and specific LAMP assay for Escherichia coli. It does not require DNA extraction and can detect as few as 10 copies. It detected all 36 of 36 E. coli isolates and all 22 urine samples (out of 89 samples tested) that had E. coli. This assay is rapid, low in cost, and simple to perform.",

author = "Joshua Hill and Shilpa Beriwal and Ishwad Chandra and Paul, \{Vinod K.\} and Aarti Kapil and Tripti Singh and Wadowsky, \{Robert M.\} and Vinita Singh and Ankur Goyal and Timo Jahnukainen and Johnson, \{James R.\} and Tarr, \{Phillip I.\} and Abhay Vats",

year = "2008",

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AU - Hill, Joshua

AU - Beriwal, Shilpa

AU - Chandra, Ishwad

AU - Paul, Vinod K.

AU - Kapil, Aarti

AU - Singh, Tripti

AU - Wadowsky, Robert M.

AU - Singh, Vinita

AU - Goyal, Ankur

AU - Jahnukainen, Timo

AU - Johnson, James R.

AU - Tarr, Phillip I.

AU - Vats, Abhay

PY - 2008/8

Y1 - 2008/8

N2 - We developed a highly sensitive and specific LAMP assay for Escherichia coli. It does not require DNA extraction and can detect as few as 10 copies. It detected all 36 of 36 E. coli isolates and all 22 urine samples (out of 89 samples tested) that had E. coli. This assay is rapid, low in cost, and simple to perform.

AB - We developed a highly sensitive and specific LAMP assay for Escherichia coli. It does not require DNA extraction and can detect as few as 10 copies. It detected all 36 of 36 E. coli isolates and all 22 urine samples (out of 89 samples tested) that had E. coli. This assay is rapid, low in cost, and simple to perform.

UR - https://www.scopus.com/pages/publications/53149112380

U2 - 10.1128/JCM.00152-08

DO - 10.1128/JCM.00152-08

M3 - Article

C2 - 18550738

AN - SCOPUS:53149112380

SN - 0095-1137

VL - 46

SP - 2800

EP - 2804

JO - Journal of clinical microbiology

JF - Journal of clinical microbiology

IS - 8

ER -

Loop-mediated isothermal amplification assay for rapid detection of common strains of Escherichia coli

Abstract

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