Loop-mediated isothermal amplification assay for rapid detection of common strains of Escherichia coli

Joshua Hill, Shilpa Beriwal, Ishwad Chandra, Vinod K. Paul, Aarti Kapil, Tripti Singh, Robert M. Wadowsky, Vinita Singh, Ankur Goyal, Timo Jahnukainen, James R. Johnson, Phillip I. Tarr, Abhay Vats

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159 Scopus citations

Abstract

We developed a highly sensitive and specific LAMP assay for Escherichia coli. It does not require DNA extraction and can detect as few as 10 copies. It detected all 36 of 36 E. coli isolates and all 22 urine samples (out of 89 samples tested) that had E. coli. This assay is rapid, low in cost, and simple to perform.

Original languageEnglish
Pages (from-to)2800-2804
Number of pages5
JournalJournal of clinical microbiology
Volume46
Issue number8
DOIs
StatePublished - Aug 1 2008

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    Hill, J., Beriwal, S., Chandra, I., Paul, V. K., Kapil, A., Singh, T., Wadowsky, R. M., Singh, V., Goyal, A., Jahnukainen, T., Johnson, J. R., Tarr, P. I., & Vats, A. (2008). Loop-mediated isothermal amplification assay for rapid detection of common strains of Escherichia coli. Journal of clinical microbiology, 46(8), 2800-2804. https://doi.org/10.1128/JCM.00152-08