TY - JOUR
T1 - Longitudinal, noninvasive monitoring of compensatory lung growth in mice after pneumonectomy via 3He and 1H magnetic resonance imaging
AU - Wang, Wei
AU - Nguyen, Nguyet M.
AU - Guo, Jinbang
AU - Woods, Jason C.
PY - 2013/11
Y1 - 2013/11
N2 - In rodentsandsomeothermammals, partialpneumonectomy(PNX) of adult lungs results in rapidcompensatory lung growth. In the past, quantification of compensatory lung growth and realveolarization could only be accomplished after killing the animal, removal of lungs, and histologic analysis of lungs at single time points. Hyperpolarized 3He diffusion magnetic resonance imaging (MRI) allows in vivo morphometry of human lungs; our group has adapted this technique for application to mouse lungs. Through imaging, we can obtain maps of lung microstructural parameters that allow quantification of morphometric and physiologic measurements. In this study, we employed our 3He MRI technique to image in vivo morphometry at baseline and to serially assess compensatory growth after leftPNXof mice. 1Hand hyperpolarized 3Hediffusion MRI were performed at baseline (pre-PNX), 3-days, and 30-days after PNX. Compared with the individual mouse's own baseline, MRI was able to detect and serially quantify changes in lung volume, alveolar surface area, alveolarnumber, andregional changes in alveolar size that occurred during the course of post-PNX lung growth. These results are consistent with morphometry measurements reported in the literature for mouse post-PNX compensatory lung growth. In addition, we were also able to serially assess and quantify changes in the physiologic parameter of lung compliance during the course of compensatory lung growth; this was consistent with flexiVent data. With these techniques, we now have a noninvasive, in vivo method to serially assess the effectiveness of therapeutic interventions on post-PNX lung growth in the same mouse.
AB - In rodentsandsomeothermammals, partialpneumonectomy(PNX) of adult lungs results in rapidcompensatory lung growth. In the past, quantification of compensatory lung growth and realveolarization could only be accomplished after killing the animal, removal of lungs, and histologic analysis of lungs at single time points. Hyperpolarized 3He diffusion magnetic resonance imaging (MRI) allows in vivo morphometry of human lungs; our group has adapted this technique for application to mouse lungs. Through imaging, we can obtain maps of lung microstructural parameters that allow quantification of morphometric and physiologic measurements. In this study, we employed our 3He MRI technique to image in vivo morphometry at baseline and to serially assess compensatory growth after leftPNXof mice. 1Hand hyperpolarized 3Hediffusion MRI were performed at baseline (pre-PNX), 3-days, and 30-days after PNX. Compared with the individual mouse's own baseline, MRI was able to detect and serially quantify changes in lung volume, alveolar surface area, alveolarnumber, andregional changes in alveolar size that occurred during the course of post-PNX lung growth. These results are consistent with morphometry measurements reported in the literature for mouse post-PNX compensatory lung growth. In addition, we were also able to serially assess and quantify changes in the physiologic parameter of lung compliance during the course of compensatory lung growth; this was consistent with flexiVent data. With these techniques, we now have a noninvasive, in vivo method to serially assess the effectiveness of therapeutic interventions on post-PNX lung growth in the same mouse.
KW - Helium
KW - Magnetic resonance imaging
KW - Neoalveolarization
KW - Pneumonectomy
UR - http://www.scopus.com/inward/record.url?scp=84887027114&partnerID=8YFLogxK
U2 - 10.1165/rcmb.2012-0332MA
DO - 10.1165/rcmb.2012-0332MA
M3 - Article
C2 - 23763461
AN - SCOPUS:84887027114
SN - 1044-1549
VL - 49
SP - 697
EP - 703
JO - American Journal of Respiratory Cell and Molecular Biology
JF - American Journal of Respiratory Cell and Molecular Biology
IS - 5
ER -