@article{a3254c709bc24209bcbdfc91ca9a9966,
title = "Longitudinal multi-omics analyses link gut microbiome dysbiosis with recurrent urinary tract infections in women",
abstract = "Recurrent urinary tract infections (rUTIs) are a major health burden worldwide, with history of infection being a significant risk factor. While the gut is a known reservoir for uropathogenic bacteria, the role of the microbiota in rUTI remains unclear. We conducted a year-long study of women with (n = 15) and without (n = 16) history of rUTI, from whom we collected urine, blood and monthly faecal samples for metagenomic and transcriptomic interrogation. During the study 24 UTIs were reported, with additional samples collected during and after infection. The gut microbiome of individuals with a history of rUTI was significantly depleted in microbial richness and butyrate-producing bacteria compared with controls, reminiscent of other inflammatory conditions. However, Escherichia coli gut and bladder populations were comparable between cohorts in both relative abundance and phylogroup. Transcriptional analysis of peripheral blood mononuclear cells revealed expression profiles indicative of differential systemic immunity between cohorts. Altogether, these results suggest that rUTI susceptibility is in part mediated through the gut–bladder axis, comprising gut dysbiosis and differential immune response to bacterial bladder colonization, manifesting in symptoms.",
author = "Worby, {Colin J.} and Schreiber, {Henry L.} and Straub, {Timothy J.} and {van Dijk}, {Lucas R.} and Bronson, {Ryan A.} and Olson, {Benjamin S.} and Pinkner, {Jerome S.} and Obernuefemann, {Chloe L.P.} and Mu{\~n}oz, {Vanessa L.} and Paharik, {Alexandra E.} and Azimzadeh, {Philippe N.} and Walker, {Bruce J.} and Desjardins, {Christopher A.} and Chou, {Wen Chi} and Karla Bergeron and Chapman, {Sin{\'e}ad B.} and Aleksandra Klim and Manson, {Abigail L.} and Hannan, {Thomas J.} and Hooton, {Thomas M.} and Kau, {Andrew L.} and Lai, {H. Henry} and Dodson, {Karen W.} and Hultgren, {Scott J.} and Earl, {Ashlee M.}",
note = "Funding Information: This project has been funded in part with Federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services under grant no. U19AI110818 to the Broad Institute, from the National Institutes of Health Mucosal Immunology Studies Team consortium under grant no. U01AI095542 to Washington University and the National Institute of Diabetes and Digestive and Kidney Disease, National Institutes of Health, Department of Health and Human Services, under Grant Number R01DK121822 to the Broad Institute and Washington University. B.S.O. was supported by grants from the National Institutes of Health, USA (nos. T32GM007067 and T32GM139774). A.L.K. was supported by grants from the National Institutes of Health, Department of Health, USA (no. R01AI165915) and the Doris Duke Charitable Foundation. This work was also supported by funds from the Center for Women{\textquoteright}s Infectious Disease Research at Washington University School of Medicine. We thank members of the Broad{\textquoteright}s Bacterial Genomics group and H. Vlamakis for helpful conversations. We thank B. Haas for assistance with PBMC RNA-seq analysis, as well as the Multi-Omics Core and Genomics Platform at the Broad Institute for sample processing and data generation. Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive licence to Springer Nature Limited.",
year = "2022",
month = may,
doi = "10.1038/s41564-022-01107-x",
language = "English",
volume = "7",
pages = "630--639",
journal = "Nature Microbiology",
issn = "2058-5276",
number = "5",
}