TY - JOUR
T1 - Longitudinal morphological and functional characterization of human heart organoids using optical coherence tomography
AU - Ming, Yixuan
AU - Hao, Senyue
AU - Wang, Fei
AU - Lewis-Israeli, Yonatan R.
AU - Volmert, Brett D.
AU - Xu, Zhiyao
AU - Goestenkors, Anna
AU - Aguirre, Aitor
AU - Zhou, Chao
N1 - Funding Information:
Work in Dr. Zhou's laboratory was supported by the National Institutes of Health (NIH) grants R01-EB025209 and R01-HL156265 . Work in Dr. Aguirre's laboratory was supported by NIH grants under award numbers K01HL135464 and R01HL151505 , by the American Heart Association under award number 19IPLOI34660342 , and by the Spectrum-MSU Foundation. The authors thank Dr. Nathaniel Huebsch and his lab for sharing the WTC hiPSC GCaMP6f cell line and lab equipment, and for valuable discussions. The authors thank Mr. James Ballard for his advice on the manuscript, and Ms. Dianne Duncan and Department of Biology at Washington University in St. Louis for allowing us to use the Leica Sp8-DIVE true spectral microscope.
Publisher Copyright:
© 2022 The Author(s)
PY - 2022/7/1
Y1 - 2022/7/1
N2 - Organoids play an increasingly important role as in vitro models for studying organ development, disease mechanisms, and drug discovery. Organoids are self-organizing, organ-like three-dimensional (3D) cell cultures developing organ-specific cell types and functions. Recently, three groups independently developed self-assembling human heart organoids (hHOs) from human pluripotent stem cells (hPSCs). In this study, we utilized a customized spectral-domain optical coherence tomography (SD-OCT) system to characterize the growth of hHOs. Development of chamber structures and beating patterns of the hHOs were observed via OCT and calcium imaging. We demonstrated the capability of OCT to produce 3D images in a fast, label-free, and non-destructive manner. The hHOs formed cavities of various sizes, and complex interconnections were observed as early as on day 4 of differentiation. The hHOs models and the OCT imaging system showed promising insights as an in vitro platform for investigating heart development and disease mechanisms.
AB - Organoids play an increasingly important role as in vitro models for studying organ development, disease mechanisms, and drug discovery. Organoids are self-organizing, organ-like three-dimensional (3D) cell cultures developing organ-specific cell types and functions. Recently, three groups independently developed self-assembling human heart organoids (hHOs) from human pluripotent stem cells (hPSCs). In this study, we utilized a customized spectral-domain optical coherence tomography (SD-OCT) system to characterize the growth of hHOs. Development of chamber structures and beating patterns of the hHOs were observed via OCT and calcium imaging. We demonstrated the capability of OCT to produce 3D images in a fast, label-free, and non-destructive manner. The hHOs formed cavities of various sizes, and complex interconnections were observed as early as on day 4 of differentiation. The hHOs models and the OCT imaging system showed promising insights as an in vitro platform for investigating heart development and disease mechanisms.
KW - Chambers
KW - Heart organoids
KW - Optical coherence tomography
KW - hPSC
UR - http://www.scopus.com/inward/record.url?scp=85126704398&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2022.114136
DO - 10.1016/j.bios.2022.114136
M3 - Article
C2 - 35325716
AN - SCOPUS:85126704398
SN - 0956-5663
VL - 207
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
M1 - 114136
ER -