TY - JOUR
T1 - Long-term hematopoietic stem cells require stromal cell-derived factor-1 for colonizing bone marrow during ontogeny
AU - Ara, Toshiaki
AU - Tokoyoda, Koji
AU - Sugiyama, Tatsuki
AU - Egawa, Takeshi
AU - Kawabata, Kenji
AU - Nagasawa, Takashi
N1 - Funding Information:
We thank Dr. Thomas N. Sato (The University of Texas) for providing the Tie2 promoter/enhancer DNA. We also thank Drs. N. Tamai and A. Myoui (Osaka University) for helpful comments and J. Yoshida, K. Matsumoto, and E. Yoshimura for technical assistance. This study was supported by a Research for the Future Program grant of The Japanese Society for the Promotion of Science, Sports, and Culture of Japan.
PY - 2003/8/1
Y1 - 2003/8/1
N2 - The physiological role of SDF-1 on hematopoietic stem cells (HSCs) remains elusive. We show that colonization of bone marrow by HSCs in addition to myeloid cells is severely impaired in SDF-1-/- embryos by a long-term repopulation assay. Colonization of spleen by HSCs was also affected, but to a lesser extent. Enforced expression of SDF-1 under the control of vascular-specific Tie-2 regulatory sequences could completely rescue the reduction of HSCs but not myeloid cells in SDF-1-/- bone marrow. SDF-1 was detected in the vicinity of the vascular endothelial cells in fetal bone marrow. SDF-1 plays a critical role in colonization of bone marrow by HSCs and myeloid cells during ontogeny, and the mechanisms by which SDF-1 functions are distinct between HSCs and myeloid cells.
AB - The physiological role of SDF-1 on hematopoietic stem cells (HSCs) remains elusive. We show that colonization of bone marrow by HSCs in addition to myeloid cells is severely impaired in SDF-1-/- embryos by a long-term repopulation assay. Colonization of spleen by HSCs was also affected, but to a lesser extent. Enforced expression of SDF-1 under the control of vascular-specific Tie-2 regulatory sequences could completely rescue the reduction of HSCs but not myeloid cells in SDF-1-/- bone marrow. SDF-1 was detected in the vicinity of the vascular endothelial cells in fetal bone marrow. SDF-1 plays a critical role in colonization of bone marrow by HSCs and myeloid cells during ontogeny, and the mechanisms by which SDF-1 functions are distinct between HSCs and myeloid cells.
UR - http://www.scopus.com/inward/record.url?scp=0042432054&partnerID=8YFLogxK
U2 - 10.1016/S1074-7613(03)00201-2
DO - 10.1016/S1074-7613(03)00201-2
M3 - Article
C2 - 12932359
AN - SCOPUS:0042432054
SN - 1074-7613
VL - 19
SP - 257
EP - 267
JO - Immunity
JF - Immunity
IS - 2
ER -