Long nerve allografts in sheep with cyclosporin A immunosuppression

Takeshi Matsuyama, Rajiv Midha, Susan E. Mackinnon, Catherine A. Munro, Pui Yeun Wong, Lee C. Ang

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


Rodent studies of nerve allografts are limited by a relatively short length of graft segment. The authors attempted to establish an outbred sheep model that would allow the study of longer, more clinically relevant nerve gaps. Using outbred ewes, two 8-cm long radial sensory nerves were grafted into gaps (5 cm) in the median nerve. Sheep received an autograft and an allograft. Four sheep were immunosuppressed with Cyclosporin A (CsA) and four were controls. Blood CsA levels greater than 1000 μg/L were obtained. Systemic immunosuppression resulted in severe opportunistic infections, and the sheep were sacrificed between 35 and 47 days following surgery. Histologically, in the autografts and CsA-treated allografts, evidence of nerve regeneration was seen. Non-immunosuppressed allografts were clearly rejected. While clear differences in the histology of experimental and control grafted nerve tissues were seen, the sheep allograft model presents considerable challenges clue to immunosuppression-related infectious complications.

Original languageEnglish
Pages (from-to)219-225
Number of pages7
JournalJournal of reconstructive microsurgery
Issue number3
StatePublished - Apr 2000


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